Determine the effect of light intensity on the rate of photosynthesis in Eledeo.

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An investigation to determine the effect of light intensity on the rate of photosynthesis in Eledeo

Introduction

The process of photosynthesis is the transfer of light energy into chemical potential energy of organic molecules.

An equation for photosynthesis is:

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Photosynthesis is vital for existence of both plants and animals, as they are dependent on energy that is provided by the sun.

'Green plants and other photosynthetic organisms have the essential role of providing the entire input of energy to an ecosystem.'ii

'Photosynthesis in plants converts light energy to chemical energy in organic molecules. Animals eat plants, obtaining some of the chemical energy in the molecules they taken in.'iii

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In photosynthesis, there are two sets of reactions involved:

-The light independent reaction (Calvin cycle), which take place in the chloroplast stroma. Its role is the fixation of carbon dioxide, which is reduced to carbohydrates, using hydrogen and water. The carbon dioxide combines with a pentose sugar to produce ribulose biphosphate (RuBP). This continues to form two molecules of a three-carbon molecule called glycerate-3-phosphate. From this, two triose molecules are created due to the presence of ATP and reduced NADP, of which some condenses to form hexose phosphates such as starch. The remainder regenerates RuBP.

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I am going to concentrate on the light dependant stage of photosynthesis as I am going to investigate the effect of light intensity on the rate of photosynthesis.

In the light dependent stage of photosynthesis, light energy is trapped. Energy from sunlight causes electrons to be emitted form the chlorophyll molecules. Some of the energy given from those electrons produces APT (Adenosine triphosphate) and some is used to produce reduced NAD. Water molecules are then split (photolysis) to produce free electrons, which are used to replace those that were emitted by the chlorophyll molecules. The hydrogen ions produced are used for the production of ATP and oxygen is given off as a waste product.

H2O --> 2H+ +2e- + 1/2O2 vi

The light energy is trapped in the lamellae by the photosynthetic pigments called chlorophylls and carotenoides. Different pigments absorb different wavelengths of light.
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The photosynthetic pigments are arranged as photo systems. A photo system is a light-harvesting cluster.

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A method of investigating the effect of light intensity on the rate of photosynthesis is by measuring the amount of oxygen evolution from aquatic plants. Aquatic plants are easier to use as the bubbles can be seen when the plant photosynthesise as the oxygen bubble is ejected. With land plants, oxygen cannot be measured, as it cannot be seen. Another accurate method to investigate the effect of light intensity on the rate of photosynthesis is by measuring the volume of ...

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