• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Discover the affects of PH on the enzyme catalase.

Extracts from this document...

Introduction

Aim The aim of this investigation is to discover the affects of PH on the enzyme catalase Introduction: Catalase- This is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous by-product of metabolism. It speeds up the decomposition of hydrogen Peroxide into oxygen and water. It is able to do this because the shape of its active site matches the shape of Hydrogen Peroxide molecule. This type of reaction where a molecule is broken down into smaller pieces is an ANABOLIC REACTION. BACKGROUND INFORMATION ON ENZYMES: Enzymes- Enzymes are complex 3-D globular proteins, some of which have other associated molecules. Enzymes are a catalyst which alters the rate of chemical reaction without itself undergoing a permanent change, and therefore can be used over and over again. Enzymes help reactions speed up which would otherwise take place very slowly. ...read more.

Middle

The rest of the variables will be kept constant and only the temperatures will be changed. Apparatus To test how the PH affects the rate of reaction, I will use the following apparatus: * Gas syringe through bung * Potato * Hydrogen Peroxide * Stop Clock * Cork borer * Scalpel * water Beaker-250 cm� * Delivery Tube * Ph 3,5,7,9 * 5 cm� or 10 cm� plastic syringe with end cut off * measuring cylinder * conical flask * White tile * wax * Test tubes * Test tube rack * Safety equipment; goggles, lab coat, gloves Alternative: Use yeast, broad beans or liver, instead of potato and apple. Method 1. Measure out 5cm3 of potato into a conical flask 2. Measure out 10 cm3 of buffer solution into a separate container and mix it with minced potato. 3. Measure out 5cm3 volume of hydrogen peroxide in syringe. 4. ...read more.

Conclusion

10.0 17.35 2.0 240 1.5 11.1 20.15 2.0 270 1.5 13.6 24.15 2.0 300 1.5 14.0 31.15 2.0 These results clarify that the optimum ph for catalase is 7 which supports my prediction. At the lowest ph 3, less oxygen was released, measuring a slower rate of reaction. My background information explains this. The enzyme don't work as it is neared extreme PHS. This is because its shape would have been change slightly; means the active site may not fit into the substrate any more. Further investigation: If this investigation were to be done again, I would take all results myself and use my own interpretation, thus gaining more reliable results. I will know how much potato to measure out for each experiment and also I will make sure that the other variables are kept constant. I have taken the same amount of each, keeping them constant. This would give me clearer results to aid me in my understanding as to how ph can affect the activity of catalase. Saira ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Biology Coursework: Investigation of How Ph Affects the Rate of Reaction of Catalase

    3 star(s)

    time * The breakdown point is where the catalase appears to have been denatured by the pH and is reacting so slowly or not at all that the time taken is taken as indefinite. ("oo"). From these conclusions, the enzyme is shown to have different rates of reaction at different pH ranges.

  2. Influence of pH on the activity of potato Catalase

    activity of potato catalase, this is because it is most effective at producing oxygen. The pH 8 in theory should be more effective than pH 7, however in the results it is the worst. This could be because the pH may have been become denatured if not kept in the

  1. What influence does pH have on the enzyme Catalase?

    This relationship of the rate of reaction increasing is not definite as the error bounds showing the range of results overlap, showing that the true rate of reaction theoretically could be the same at pH 5, 6, 7 and 8.

  2. Investigation on how pH affects free and immobilised catalase enzymes.

    The induced fit theory is that as the enzyme and substrate approach each other it under goes a recognition process of conformational change in which the active site changes to fit the incoming substrate. And as the substrate binds to the active site creating a substrate enzyme complex the binding

  1. Investigation on how pH affects free and immobilised catalase enzymes.

    It works inside the cells of both animal and plants cells like in the liver for animals and potato cell for plants. It breaks down hydrogen peroxide in the cells that it is present in; this makes it important, as hydrogen peroxide is a very poisonous substance produced by a lot of the chemical reactions in the body.

  2. In this investigation we are going to investigate how temperature affects the activity of ...

    for the hydrogen peroxide to collide with so the rate of reaction will increase (collision theory). The concentration could be varied by using a different potato, a different aged potato, a different sized potato, and a different type of potato or by using the potato with or without its skin.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work