Does caffeine affect heart rate

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Does caffeine affect heart-rate?

In this investigation I intend to find out if caffeine has an affect on heart rate. As an alternative to using human subject, who might previously have had a high tolerance to caffeine or possibly will have taken a dose of caffeine that day making the tests unfair, instead I will use Daphnia, a crustacean. They have no contact with caffeine making it fair. It will be simpler to examine as it has a translucent body, making it able to see the heart beating under a microscope. I will be making this a "blind study" to avoid bias results, so I will be oblivious to what the solution consists of. To make this experiment accurate and reliable, I will be using graphs and tables to show information about my results; I will use the graphs to compare the results with the control. I will also include a write up about the procedure of the experiment.

Caffeine

Caffeine acts as a stimulant drug in humans and other animals. Caffeine is found in different quantities in the beans, leaves, and fruit of some plants. It is mainly consumed by humans in hot drinks, taken out from the beans of the coffee plant and the leaves of the tea bush. In humans, caffeine is a CNS stimulant, having the effect of temporarily keeping drowsiness at bay and restoring alertness. Beverages containing caffeine, such as coffee, tea, soft drinks and energy drinks enjoy great popularity. Caffeine is the world's most widely consumed psychoactive substance

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Daphnia (water flea)

Daphnia are small crustaceans. Daphnia are one of a number of small aquatic crustaceans commonly called water fleas because of their saltatory swimming style. They live in a variety of aquatic environments, ranging from acidic swamps to freshwater lakes, ponds, streams and rivers.

Hypothesis

I consider that a Daphnia subjected to a caffeine solution will show a raise in the average heart rate, this rise in heart rate should correspond with the concentration of caffeine in a given solution. My theory can be backed up well, because caffeine is a stimulant that speeds up the body ...

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