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Does temperature affect how well enzymes work?

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Does temperature affect how well enzymes work? Aim To investigate whether the enzyme Catalase is affected by different temperatures by using catalase from potato at a range of different temperatures and to measure how enzyme has worked by measuring the amount of oxygen collected Introduction * Living cells continuously carry out chemical reactions and as a by-product produce hydrogen peroxide. It is a toxic substance which can destroy the cells if not immediately broken down. * This breakdown is carried out by the enzyme catalase, which is produced in all living cells. * Catalase breaks down the hydrogen peroxide into water and oxygen making it harmless to the cells. Hydrogen CATALASE Peroxide Water + oxygen 2H2O2 2H2O+ O2 Therefore enzyme catalase is the catalyst. Hydrogen Peroxide is the substrate. Catalase is the fastest enzyme known. When it comes into contact of Hydrogen peroxide, frothing shows rapid production of oxygen. . Catalase is to break down. This is what enzymes do. Catalase is the name of the enzymes I am studying. ...read more.


* Concentration of hydrogen peroxide * Source of potato will be the same or the test would be unfair. How does the enzyme work? Thousands of chemical reactions take place in living cells to keep them alive. Enzymes make these reactions happen much faster. Chemicals which speed up reactions are called catalysts, and since enzymes work in living cells, they are called biological catalysts. Enzymes work on substances called substrates. The substrates attach to the active site of same shape and thus a reaction takes place. All enzymes have some important properties: * They are all proteins. Hence they have a specific shape. * Specific enzyme controls one particular reactions (it is specific) * They can be used over and over again. Hence they are economically viable. * They are affected by changes in temperature, and each will have its own optimum temperature. * They are affected by changes pH (how acidic or alkaline) and each will have its optimum pH. * They can be destroyed (denatured) by heating (boiling). ...read more.


This would reduce the amount of O2 in the measuring cylinder. 2. Some of the grated potato produced quite a lot of foam which rose in the delivery tube with the oxygen. This made it difficult to read the volume of oxygen in the measuring cylinder. 3. My delivery tube was made with 2 parts joining them with a rubber tube. During some experiments this came apart and might have affected my experiment. 4. In my experiment there were some results (anomalies result(s) highlighted with black colour ) Temperatures in � C Experiment 1 Experiment 2 (repeat) Average Experiment 3 (teacher's result) 0 5 30 17.5 6 24 22 40 31 13 40 35 45 41 41 60 10 10 31 31 80 3 3 3 5 This is out of line with my prediction. I expected the volume of oxygen to be the greatest at the range of about 40� C and lower at the cold and hot temperatures. It was but 30 ml of 02 is quite high. How ever my average results followed same pattern as my teacher's result, and so safely I can say that my results match with my predictions ?? ?? ?? ?? GCSE Biology coursework. - 1 - By Rushal Raghwani 11P ...read more.

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