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Does the Concentration of CuSO4 Affect the Germination of Barley Seeds?

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Does the Concentration of CuSO4 Affect the Germination of Barley Seeds? Investigation Analysis When looking at the data collection sheet it can be seen that as the concentration of CuSO4 increases, the percentage of seed germination decreases. This already shows a clear sign that enzyme inhibition has occurred. Looking at the "Average Length of Roots" graph, it can be seen that the concentration of CuSO4 does inhibit the growth of roots significantly because the average root length drops sharply after the 0% solution. However some of my results nay have been anomalous because the decline is far too steep to draw any reliable conclusions. When comparing this graph to the "Average Length of Shoot" graph, it can be seen that the average shoot length for each concentration depreciates at a steadier rate; perhaps then, heavy metal ions affect enzymes that are responsible for root growth more than they affect those that are responsible for shoot growth. ...read more.


are indirectly affected by the heavy metal ions, which change the shape of the active site so that the enzyme cannot properly interact with the substrate. This is known as the allosteric affect. Therefore, the more heavy metal ions (higher concentration) the more enzyme ions are affected resulting in less successful enzyme-substrate collisions. Evaluation Within this investigation there were many limitations which may have hindered results and caused anomalies. Firstly, when we measured out the concentrations of coppers sulphate solution, there is already a degree of error of +/- 0.5mm. This may have caused my results to be misleading and encourage false results. However, my results seem to be pretty reliable and when comparing with other members of my class, we have similar results in context to the experiment. Using a burette or another more accurate measuring device for future investigations could prevent or reduce the margin of error. ...read more.


So, time could be another controlled variable on the investigation. Another factor is human error, such as measuring the length of the shoots and roots etc, which has a margin of error of 0.5mm for length and 0.5g for mass. Although these would only be small inaccuracies, the data would still be affected and allow for inaccurate conclusions to be drawn. Doing this investigation again, I would use much more accurate measuring equipment such as scales which are more accurate or maybe just a ruler with smaller scales. Finally, when we actually sealed the test tubes into the bag, we blew air into it. So we actually blew in CO2, which may have helped germination to occur as plants take in CO2. This may have caused varied results among the seeds because some seeds may have been subjected to more CO2 than others, causing anomalous, inaccurate data. Doing the investigation again, we could use a more suitable method of maintaining the air concentration within the sealed bag. These factors may have contributed to the varied result for each repeat. Adil Hussain Yr12IB Bio Coursework ...read more.

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