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Does the increase in surface area of a product affect the overall rate of oxygen production/catalase reaction.

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Introduction

Does the increase in surface area of a product affect the overall rate of oxygen production/catalase reaction. Aim: To see if an increase to the surface area effects the overall rate of catalase absorption. Prediction: I predict that as the surface area increases so will the overall rate of oxygen production. This is because when a substrate (Hydrogen Peroxide) reacts with an enzyme (catalase) it is changed into a product (oxygen) (See diagram 2). The equation for this is hydrogen peroxide (H2O2)= oxygen + water (O+ H2O). An enzyme works when the surface are of an enzyme is cut. The substrate enters the enzyme through the cut cell (See diagram 1). If an enzyme has a larger surface more of an enzyme is released therefore increasing the rate of oxygen production. Fair test: To make the experiment fair and useable in the future we needed to make sure we obtained valid results. There are some factors which will affect the results, these are: Temperature; we made sure that the temperature wasn't effected by completing the experiment over the course of one day at room temperature. An enzyme works at an optimum temperature of 37�c (body temperature), if an enzyme is heated too more than 40�c it becomes denatured (killed), and if it is cooled to less than 37�c it works slower and therefore becomes less effective. ...read more.

Middle

2. Using the cork borer I cut three cylinders of potato at 4cm long each (using ruler). 3. I filled up the syringe to the 10ml3 with hydrogen peroxide. 4. I placed one of the three potato cylinders into the conical flask and replaced the bung securely, so allowing no air to enter into the flask. 5. I then pushed the hydrogen peroxide into the conical flask and didn't take the syringe out (so I didn't let any air in),I timed a minute (to see how much oxygen was produced) and recorded the results the table. 6. I repeated this two more times and took an average (see table). 7. I then took three results each for when there was two pieces, four pieces, eight pieces and sixteen pieces and took there averages Results: To make these results more accurate we have measured the radius of the cork borer (5mm) and the height (40mm) of the cylinder. We have done this to work out the surface area. The equation for surface area of a cylinder is 2?rh + 2?r2. No of pieces (potato) Surface area (cm2) Volume of oxygen produced in 1 min (cm3) Average (cm3) ...read more.

Conclusion

I would also take this chance too see what went wrong in the first line of results and too do my best to prevent this from happening again, when redoing this practical. The factors which effected the results are accuracy and reliability. The points of the fair test effected reliability (temperature, same potato, same people -to read results-, same equipment, same volume and concentration of hydrogen peroxide, same pH). This affected the range of results (24.3- 9.0 =15.3), this is making the range larger (it should be roughly 6.0) therefore decreasing the accuracy of the results. The equipment used effected the accuracy, we used a ruler and scalpel to measure the potato to one decimal place (of length), and used the same pieces of equipment each time we read results. Every time we used the same person to read results so that the margin of human error was the same each time. Each time we syringed hydrogen peroxide into the conical flask we displaced an air bubble, which was at the top of the syringe. I believe that over a longer course of time I could improve the method by making it more detailed, which would therefore increase the accuracy of the results. ...read more.

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