• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Effect Of Substrate Concentration On The Activity Of Catalase

Extracts from this document...

Introduction

��ࡱ�>�� BD����A�������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������5@ ��0�5bjbj�2�2 (P�X�X�*���������������llll8� �TlSv�������$�R ����� �������������� `F--,��lp��#0S� � ��t"J���� �� ����Effect Of Substrate Concentration On The Activity Of Catalase Effect Of Substrate Concentration On The Activity Of Catalase AIM This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. PREDICTION I predict that as the substrate concentration increases, the rate of reaction will go up at a directly proportional rate until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point is reached, then adding extra substrate will make no difference. The rate steadily increases when more substrate is added because more of the active sites of the enzyme are being used which results in more reactions so the required amount of oxygen is made more quickly. Once the amount of substrate molecules added exceeds the number of active sites available then the rate of reaction will no longer go up. This is because the maximum number of reactions are being done at once so any extra substrate molecules have to wait until some of the active sites become available. BACKGROUND INFORMATION Enzymes such as Catalase are protein molecules which are found in living cells. ...read more.

Middle

47.3 18.4 17.3 14.5 10.6 9.7 Time Taken (Test 2) 43.3 19 16.7 14.9 11.2 10 Time Taken (Test 3) 52.2 17.2 18.5 11.2 8.6 7.8 Average of the Tests 47.6 18.2 17.5 13.5 10.1 9.2 Rate=30/Average (Cm3/second) 0 0.63 1.65 1.71 2.22 2.97 3.26 All the times are in seconds. The average results are all written down to one decimal place because although the stopwatch gives results to two decimal places it is impossible to get accurate times to two decimal places due to the fact that our reaction times are not fast enough to stop the stopwatch precisely. I then worked out the rates of the reactions with the equation from Rate=30/Average Time From these rates I was able to plot a graph of the rate of reaction against concentration of Hydrogen Peroxide. CONCLUSION When the concentration of Hydrogen Peroxide is increased, the rate of reaction increases at a directly proportional rate until the concentration of Hydrogen Peroxide reaches about 16%. If you double the concentration of Hydrogen Peroxide then the rate of reaction doubles as well. When the concentration is doubled from 8-16% the rate goes up from 1.65-2.97 Cm3 Oxygen produced per second, which is an increase of 1.8 times. ...read more.

Conclusion

This document was downloaded from Coursework.Info - The UK's Coursework Database - http://www.coursework.info/ This document was downloaded from Coursework.Info - The UK's Coursework Database - http://www.coursework.info/ r�o��2�2m3n3�3�3Q4R4�4�45565�5�5�5�5������۳ۦÛ��ۦ��h*qh*qOJQJh*qh*qCJOJQJ%h*qh*qOJQJfHq� ����)h*qh*qCJOJQJfHq� ����h*qhL>�h*qh*qmHsHh*qh*qmHsH hL>�h*q>?@A������� & ' ( ) J K L M L M N O f �����������������������������gdL>��2�5��f g h i F G H I opqr���������������������������������������������gdL>�-.=>JK^_no{|������������&'(�����������������������������gdL>�()HIJK ��������)*67?@KL�����������������������������gdL>�LWXabcd�������78TUab������������������������������������gdL>���������}~� ������OP���������������������������������gdL>����WXYZ[\� � � � � � � � S!T!g!h!i!j!u!v!w!x!�$�����������������������������gdL>��$�$�$�$''''�(�(�(�(�(�(�(�(X*Y*Z*[*�+�+�+�+�-�-�-�-W.X.�����������������������������gdL>�X.Y.Z.d.e.f.g.�/�/�/�/�/�/�/�/1111 2 2�2�2n3o3p3q3�3���������������������������$a$gd*qgdL>��3�3�3�3R4S4T4U4�4�4�4�465758595�5�5�5�5�5�5���������������������gdL>�$a$gd*q$a$gd*q&1�h:pL>���/ ��=!�'"�'#��$��%��D@�D NormalCJ_H aJmH nHsH tHDA@�D Default Paragraph FontRi�R Table Normal�4� l4�a� (k�(No ListDZ@�D L>� Plain TextCJOJQJ^JaJ4@4 *qHeader ���!4 @4 *qFooter ���!`�o"` *qwatermark header$a$CJOJQJfHq� ����N�o2N *qwatermark footer$a$ CJOJQJ�-P���� ���z� ���z� ���z� ���z� ���z� ���z� ���z��K T��'�-�>�%r�V�:���5f (L���$X.�3�5 !"#$%&'�5r�o��*�*o+q+�+�+S,U,�,�,7-9-�-�-�*�-�*�-��alex�*qL>��@�*�D�-P@��Unknown������������G��z ��Times New Roman5V��Symbol3&� �z ��Arial7&�� �VerdanaG5�� �����h�MS Mincho-�3� fg?5� �z ��Courier New"1���hF �FF �FF �F�R$d��R$d�$�������4�+�+3�� H�?������������������L>���=Effect Of Substrate Concentration On The Activity Of CatalaseTCoursework.Info Coursework - http://www.coursework.info/ - Redistribution ProhibitedTCoursework.Info Coursework - http://www.coursework.info/ - Redistribution Prohibitedalexalex�� ��Oh��+'��0����HX� $0 L X d p|����>Effect Of Substrate Concentration On The Activity Of CatalasetUCoursework.Info Coursework - http://www.coursework.info/ - Redistribution ProhibitedualexewoUCoursework.Info Coursework - http://www.coursework.info/ - Redistribution Prohibitedu>Downloaded from Coursework.Info - http://www.coursework.info/is Normal.dotfalexl.d2exMicrosoft Word 10.0@@�-#,��@�-#,��@�-#,���R$�� ��Õ.��+,��D��Õ.��+,���X���H����� ���� � :�UCoursework.Info Coursework - http://www.coursework.info/ - Redistribution ProhibitedtUCoursework.Info Coursework - http://www.coursework.info/ - Redistribution ProhibitedtUCoursework.Info Coursework - http://www.coursework.info/ - Redistribution Prohibitedt�+�d�+A >Effect Of Substrate Concentration On The Activity Of Catalase Titled@���+K_PID_LINKBASE CopyrightDownloaded FromCan RedistributeOwner�A4http://www.coursework.comcoursework.comehttp://www.coursework.comioNo, do not redistributecoursework.com/ !"#$%&'(����*+,-./0����2345678����:;<=>?@��������C����������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������Root Entry�������� �F�.-,��E�1Table��������)WordDocument��������(PSummaryInformation(����1DocumentSummaryInformation8������������9CompObj������������j������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������ ���� �FMicrosoft Word Document MSWordDocWord.Document.8�9�q ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Factors Affecting the Rate of Catalytic Decomposition of Hydrogen Peroxide.

    These H202 particles collide with the Mn02 particles in order to make the Hydrogen Peroxide decompose. When there are twice as many H202 particles, there is twice the probability of a collision between H202 particles and Mn02 (catalyst) particles, and therefore twice as many collisions should occur.

  2. The effect of aspirin on the action of bovine liver catalase

    Contact with skin: Wash using soap and water o Ingestion: Seek medical advice Liver solution may be flushed down the sink with water. Any remaining liver can be wrapped in a polythene bag before being disposed of by the bin.

  1. The Decomposition of H2O2 using Catalase, in yeast as a catalyst.

    Evaluation My results agreed with my prediction and scientific theory, they also showed clear trends. I do not think there were any significantly anomalous results because the repeats meant that if one set of results was significantly different to the other two sets of results at that temperature, it did

  2. An Investigation of the Effect of Copper Sulphate on Catalase Activity.

    46.1 1.0 1 48.5 48.3 48.1 48.0 47.9 47.8 47.6 2 47.2 47.0 46.8 46.7 46.6 46.5 46.4 3 46.1 45.9 45.7 45.5 45.4 45.3 43.2 Table 4. A table to show the mean amount of oxygen produced (in cm3, to 2 d.p.)

  1. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Decrease in the rate of reaction as inhibiter is added. pH Each enzyme has a specific pH concentration level. If this is somewhat altered, the enzyme can denature as the structure of the enzyme can alter too, therefore; its active site can change so dramatically that substrates can longer bind to it.

  2. How Temperature Affects Catalase Activity.

    Every enzyme has an active site where the substrate molecules join to the enzyme molecules to form a product molecule. Every enzyme has a specific shape for a specific substrate to fit into and start functioning; this is why enzymes do not work in conditions that denature them.

  1. Enzymes - show how substrate concentration affects the rate of reaction for an enzyme ...

    As result of this particles move faster and faster moving particles collide more frequently. This will result in more product, water and oxygen produced in a given time. Hence rate of reaction will increase as temperature increases. Use a thermostatically controlled water bath to maintain the constant temperature of 35�C

  2. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    pipette has been washed out so that the sodium carbonate does not react with anything before the experiment begins, this will result in accurate and precise results. 8. Measure out 10ml of lipase into test tube labelled lipase using pipette again make sure it has been rinsed out of any chemicals.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work