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Effect of temperature on catalase

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Aim and Hypothesis The investigation that we have chosen to do is how the effect of temperature affects the rate of reaction of hydrogen peroxide to water and oxygen using the enzyme catalase. I predict that the higher the temperature the faster the rate of reaction will be and the more oxygen there will be given off. I've based this prediction on kinetic theory (every 10 degree rise in temperature the rate of reaction doubles.) This is because the substrate will lock on twice as fast, as it is travelling twice as fast. To back up this prediction a pre experiment was carried out, one result was obtained, using this result here is a table of predicted values: Temp of 2H2O �C Amount of Oxygen produced CM3 7 3.5 17 7 27 14 37 28 47 56 57 112 67 0 - Denatured Red = initial experiment average. ...read more.


Using my pre experiment starting point I will now go on to prove or disprove my hypothesis. Methodology The Experiment will be repeated at various temperatures to get a wide range of results. To record the volume of oxygen being produced, the amount of bubbles being given off could be recorded. However this would be a very inaccurate method, as far too many bubbles would be given off to be able to count, there would also be a wide margin for human error. A different method could be to measure the foam produced by the bubbles in the test tube, but this also wouldn't give precise results, as the bubbles near the top of the foam would burst, giving an inaccurate measurement. The chosen method was to attach a stopper over the test tube containing the potatoe and 2H2O. ...read more.


* Type of potatoe * Temperature Chosen Variable * Where the tube of Potatoe is taken from * Different light intensity Things kept the same to ensure a fair test * Surface area of Potatoe 230 cm2 ( rounded to 3 significant figures) * Volume of Peroxide 6 ml3 * Time experiment was left to run 150 Seconds The experiment had to be running for a reasonable amount of time to get good results, but there was a time limit, of getting the experiment finished within the lesson so 150 seconds was chosen We couldn't use vast amount of peroxide because we only had regular test tubes, so 6 ml3 was used. The same applies for the surface area of potatoes; it had to be to scale with the volume of peroxide, so 230 cm2 was used. At each temperature the experiment was repeated 3 times to get an average, this gave a more accurate result. ...read more.

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