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Effect of Temperature On the Movement of Pigment Through Cell Membrane

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EFFECT OF TEMPERATURE ON THE MOVEMENT OF PIGMENT THROUGH CELL MEMBRANE INTRODUCTION: AIM: The aim of experiment is to prove that temperature effects on a proteins in cell membranes so that pigment can pass through them HYPOTHESIS: On higher temperatures proteins in membranes denature so there is no barrier to prevent the passage of large molecules THEORETICAL BACKGROUND: Certain chemicals and treatments, such as ethanol or high temperatures, can destroy the partial permeability of cell membrane. The membranes are still present but behave as if holes have been punched through them and they no longer provide barrier to the passage of large molecules such as sucrose. High temperatures and alcohols denature membrane proteins and increase fluidity of membrane lipids; alcohols at high concentrations can also dissolve lipids. In beetroot cells the red anthocyanin pigment occurs in the vacuoles. Each vacuole is surrounded by a tonoplast membrane and outside of it, the cytoplasm is surrounded by the plasma membrane. ...read more.


* Repeat the procedure for all temperatures. Keep the volume of water about 200 cm3 in the beaker by removing some volume of water from it. If you accidentally lower the temperature too much, heat the baker a little bit. * Leave the cylinders in the test tubes for exactly 30 min. When 30 min passes shake the test tubes and then remove the beetroot. * When all beetroots are removed samples of coloured water should be put in the colorimeter machine and values should be recorded. The smaller the value is the darker is colour of solution, therefore larger amount of pigment left the cell. RESULTS TEMPERATURE COLOUR INTENSITY OF WATER AFTER HEATING 85 13.8 80 14.8 75 17.8 70 18.5 65 35.5 63 39 60 40.6 55 42.6 50 45.4 45 63 GRAPH ANALYSIS The graph shows a general slope downwards meaning that grater value of Y implicates smaller value of X. ...read more.


Solution is to use more sensitive equipment or to get colorimeter checked because it could be broken. * Fifth problem is in humane nature. During the test we had a good time so occasionally we forgot to take the beetroot out for few seconds longer than it should be. We didn't pay all attention to professor so we forgot to shake test tubes before we took the beetroot out. Also, I didn't take a paper with me during the colour test so when I got result, occasionally I would forget what it was while I was going back to my seat. Also there was some noise in the classroom so I could misunderstand my partners in the experiment when they came with result. * To be sure in experiment results the best thing is to repeat it at least once to increase the reliability of them. Now when I know the whole procedure of experiment, results in repetitive experiment should be more accurate. Also this is good background to compare how different concentrations of ethanol effect on the membrane permeability. ...read more.

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