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Effect of temperature on the rate of reaction of oxygen given off in the reaction between Hydrogen peroxide and catalase

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Introduction

Effect of temperature on the rate of reaction of oxygen given off in the reaction between Hydrogen peroxide and catalase Aim: To find out how the concentration of hydrogen peroxide changes the amount of oxygen produced when added to a potato chip. Background Information: There are many ways in which the amount of oxygen produced could be altered these include: the temperature of the hydrogen peroxide, the concentration of hydrogen peroxide, surface area of the potato chip or the amount of each substance used. I have chosen to use the temperature of hydrogen peroxide as my experiment. Concentration of hydrogen peroxide: if the particles are in low concentrations there will be few collisions and as the concentration increases so does the chance of collisions. Hydrogen peroxide is also a very dangerous and should be handled with care. It looks like water but is a bleach. It is broken down by a catalyst into water and oxygen: H�O� --> 2H�O + O� Catalase is the enzyme in this reaction. An enzyme is a catalyst that helps speed up reactions. It does this by lowering the activation energy needed to trigger the reaction. For an enzyme to connect well with the substrate the molecules must bind like a lock and key. However if changes to the environment are made then the enzyme can be changed and the lock and key can be affected. ...read more.

Middle

The results will be recorded in a table and on a graph and I will repeat each experiment once. To make sure it is a fair test I will only change the temperature and keep the other variables constant because if there are any slight changes it could affect my results. Variables: Independent - Temperature Dependant - Rate of reaction, amount of oxygen Uncontrolled - Light Controlled - Concentration of hydrogen peroxide Amount of potato Volume of hydrogen peroxide Safety: Whilst conducting the experiment I will: * Wear goggles * Tuck tie in * Take extra care when handling hydrogen peroxide as it is a bleach and can burn. Results: Experiment 1 Temperature(�C) 20 30 35 40 50 Amount of ml's filled Time (secs) 1 22 18 17 17 15 2 43 35 32 31 27 3 65 53 48 43 38 4 89 71 64 55 51 5 111 84 81 68 62 Experiment 2 - Repeat Temperature(�C) 20 30 35 40 50 Amount of ml's filled Time (secs) 1 22 19 16 15 14 2 41 36 32 29 27 3 62 56 49 40 39 4 83 75 66 52 50 5 103 90 83 64 61 Analysis: From my results one can clearly see that as temperature increases the rate of oxygen given off also increases. ...read more.

Conclusion

To improve this experiment we could use better equipment like a water bath that keeps the temperature constant or made sure each piece of potato chip had the same surface area. My results seemed fairly accurate apart from a slight anomaly at 30�C on the first experiment where it took 84 seconds to fill 5ml along side in experiment two at 35�C it took 83 seconds. Almost the same time at different temperatures which could be due to the temperature fluctuating whilst the experiment was happening. I believe that my results are good enough to prove that temperature has a great effect on the rate of oxygen being given off in this experiment and that it will increase with temperature increase. However I would need to do more experiments and more accurate ones to prove that rate of reaction will double with every 10�C increase in temperature. The results are reliable in with what they show us as the procedure was fairly good and no major anomalies or differences occurred. To further this experiment and find out more evidence we could do more repeats and use a greater range, possibly from 0�C to 100�C. We could also look at changing different variables like changing the concentration of the hydrogen peroxide and not just the temperature to see if that affects the results. If the surface area of the potato chips was altered we could see how this affects results and also light intensity. ...read more.

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