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effects of substrate concentration on the activity of the enzyme catalase.

Extracts from this document...

Introduction

Investigate the effects of substrate concentration on the activity of the enzyme catalase. To explain this, catalase is an enzyme found in potato cells, catalase breaks down the substrate Hydrogen-Peroxide into water + oxygen. Hydrogen Peroxide itself is a waste product found in potatoes and other fruit + vegetables. When I do this I will see with different concentrations of the substrate and see how quick the enzyme catalase works on it. The four things that affect the rate at which the substrate and enzyme would react are as follow:- * Susceptibility of temperature change. * Susceptibility to PH changes. * Enzyme concentration. * Substrate concentration. Now I will explain each one of these in detail and how they can change the rate of reaction in many different ways. Susceptibility of temperature: - Chemical reactions can be speeded up by heating the reactants, this makes molecules speed up and have sufficient energy for more successful collisions. If the temperature is too high the enzyme would vibrate and it would denature because the active-site would change shape. Susceptibility of PH changes: - Enzymes can be denatured by changes in PH. ...read more.

Middle

Time Taken for 5cm� Gas to be Produced(2nd) 100 10 0 2 80 8 2 2 60 6 4 2 40 4 6 2 20 2 8 2 0 0 10 2 My Results and Evidence I carried out the investigation safely and accurately cleaning out every beaker I use more then once, and I kept it a fair test by not changing the volume of catalase. I collected enough evidence and recorded the experiment and making sure I repeated it. These are the results I recorded from the experiment:- Percentage Concentration % Volume Of H�O�(cm�) Volume of Water (cm�) Volume Of Catalase (cm�) Time Taken for 5cm� Gas to be Produced(1st) Time Taken for 5cm� Gas to be Produced(2nd) Average Time Taken (secs) 100 10 0 2 7 9 8 80 8 2 2 11 14 12.5 60 6 4 2 19 24 21.5 40 4 6 2 28 32 30 20 2 8 2 38 34 36 0 0 10 2 (too long) (too long) N/A I do not need to repeat my investigation as I can clearly tell that the results are somehow similar in that they go down in values, I did the experiment making sure the temperature stays the same and the volume of catalase and none of the other variables change as I have done all these molars on the same day. ...read more.

Conclusion

Instructions For Carrying Out The Experiment * Set up the apparatus as shown. * Setup a separate layout, of Bunsen burner, tripod and heatproof mat, measuring different temperatures, and measuring with a thermometer. * Use different temperatures of Hydrogen Peroxide, 40�c, 50�c, 60�c, 70�c, 80�c, 90�c, measure out the same concentration and add into test-tube.(So I would need to do 6 experiments with each with different temps.) * Then add no water to the 10cm�. But when you do experiment with 8cm� add 2cm� of water to make the volume the same as equal to 10cm�. And the same for the other temperatures. * The drop in the 2cm� of catalase to the solution. * When you drop catalase, start stop-clock instantly. * Observe ruler and test-tube, and when the solution creates 5cm� of gas stop the clock and record your data for the 6 experiments in a table which looks like this :- Temperature Percentage Concentration % Volume Of H�O�(cm�) Volume of Water (cm�) Volume Of Catalase (cm�) Time Taken for 5cm� Gas to be Produced(1st) Time Taken for 5cm� Gas to be Produced(2nd) Average Time Taken (secs) 40�c 100 2 50�c 80 2 60�c 60 2 70�c 40 2 80�c 20 2 90�c 0 2 ...read more.

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Here's what a teacher thought of this essay

4 star(s)

This is a good report that includes a lot of detail.
1. The introduction, hypothesis and variable sections are very well written.
2. The preliminary investigation is relevant and sets a good basis for the rest of the investigation.
3. The conclusion is concise and includes data to back up the pattern.
4. There is repetition is several places that needs to be removed.
5. The evaluation contradicts itself in several places but does include good suggestions for improvement.
6. The report itself lacks structure and would benefit from subheadings.
**** (4 stars)

Marked by teacher Luke Smithen 24/04/2013

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