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Enzyme Activity and the Decomposition of Hydrogen Peroxide.

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Introduction

Enzyme Activity and the Decomposition of Hydrogen Peroxide Introduction We are going to investigate how the rate of reaction changes as the volume of hydrogen peroxide goes down and the volume of water goes up and the solution will be less concentrated each time due to the amount of water diluting it. The formula for Hydrogen Peroxide H2O2. Hydrogen Peroxide is a toxic liquid produced in the liver, and is poisonous to the human body. If the hydrogen peroxide levels build up within the body it is extremely dangerous to the person's health. Because the substance is a poison it has to be broken down. When it is broken down naturally it becomes H20+O2, but when a catalyst is used the formula turns into 2H2O 2H2O+O2 to make it balanced. A catalyst is used to make the reaction speed up. This is because the reaction to remove the hydrogen peroxide would take too long naturally and so more hydrogen peroxide builds up before it can be broken down. The biological catalyst is called an enzyme. Enzymes are large proteins that speed up chemical reactions. ...read more.

Middle

We then measured in a glass cylinder the amount of hydrogen peroxide need for example 20ml. 3. Then we would measure out the required amount of water for the experiment concerned for example 30ml. 4. After that we then filled the collection tube up with water and placed it upside-down in the bath. 5. We then put the hydrogen peroxide and the water together in the conical flask with the yeast and put the bung on securely. 6. Then we made sure that the connection with the collection tube and conical flask were connected properly and measured the how long it took for the volume of oxygen to reach 50cm3 taking the reading at 10 cm3 intervals. Safety During the experiment we must make sure that all of the conditions that we are working in are safe and could not be of any danger to any one. This is of great importance to the experiment and others around me. To keep the experiment safe we will: * Wear safety goggles * Ensure any spillages are cleaned up properly * Careful of where I put my hands while doing this experiment as the solution is a hazard * Make sure ...read more.

Conclusion

Volume of H2O (cm3) Volume of O2 (cm3) Time (mins) T1 T2 T3 5 45 10 0.33 0.52 0.51 20 0.52 1.22 1.14 30 1.16 1.56 1.46 40 1.42 2.38 2.18 50 2.16 3.30 3.03 Averages 25H2O2 and 25H2O Volume of O2 (cm3) 10 20 30 40 50 Time (mins) 0.07 0.12 0.16 0.22 0.27 20H2O2 and 30H2O Volume of O2 (cm3) 10 20 30 40 50 Time (mins) 0.09 0.19 0.27 0.33 0.4 15H2O2 and 35H2O Volume of O2 (cm3) 10 20 30 40 50 Time (mins) 0.08 0.20 0.26 0.34 0.41 10H2O2 and 40H2O Volume of O2 (cm3) 10 20 30 40 50 Time (mins) Volume of O2 (cm3) 10 20 30 40 50 Time (mins) Evaluation From my results, they tell me that my hypothesis was correct so therefore the results collected were fairly reliable. Throughout the investigation we had to measure and record the results accurately which I think was achieved to a great success. If I was to do the experiment again I would measure the results more accurately so to ensure more specific results. I would also repeat each measurement more times so I could also gain more specific average and I would also use smaller values to get a more detailed view of my results. Stephanie Glenny 11LD ...read more.

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