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Enzyme Investigation. How to find the best concentration of hydrogen peroxide that will react with the catalyse enzyme source the best? How to find the best PH buffer for the reaction to take place?

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Introduction

Enzyme investigation Introduction: An enzyme is a biological catalyst used in our body's to speed up chemical reactions, in our case the enzyme was catalase and act by breaking up the hydrogen peroxide source. There are a variety of different chemicals or aspects that can affect the productivity of the enzyme for example the saturation point; this is the point where the reaction involving the enzyme slows right down to a stop and can occur if the enzyme denatures usually by heat. There are always a lot of potential variables but we tried to control them as much as we possible, only changing the independent variable looking at the effect of this change on the dependent variable. After this, the results showed the predicted effect allowing us to form a conclusion and right any noticeable patterns or unexpected outcomes. Aim: How to find the best concentration of hydrogen peroxide that will react with the catalyse enzyme source the best? How to find the best PH buffer for the reaction to take place? Pre-test equipment: * Gas syringe- to measure the oxygen to the nearest cm� (dependent variable) the oxygen produced was the outcome that we decided to look at to see what was the best concentration this changed for every different concentration. * Delivery tube- to let the oxygen pass through into the gas syringe * Conical flask- to hold the substances * Hydrogen peroxide(10 volume)-to react with the catalyse (controlled variable) we measured out the same volume of hydrogen peroxide only changing the amount mixed with the water leading to a fair test with reliable results. * Balance- to weigh the liver to the nearest hundredth of a gram * Weighing boat- to hold the liver on the balance * Liver- source of catalase (controlled variable) we weighed this out trying to get as close to 1 gram as possible to the nearest hundredth of a gram we gave an error bar of 0.03 of a gram different either way this resulted in a very accurate and controlled test and results. ...read more.

Middle

We timed and recorded at the same time for each test allowing us to have accurate and reliable results. Concentration (Water/hydrogen peroxide) Time (seconds) Volume of Oxygen produced (cm�) Amount of H�O� (%) Amount of H�O (%) Amount of H�O� (ml) Amount of H�O (ml) 30 60 90 120 150 180 210 240 100 0 20 0 300 332 348 354 355 356 356 357 98 130 147 159 166 171 173 174 104 138 156 167 179 192 201 212 80 20 16 4 165 206 236 255 271 282 290 293 83 89 93 103 119 136 154 176 85 92 97 101 124 145 169 181 60 40 12 8 77 92 117 138 155 166 173 178 38 46 54 60 66 71 77 83 47 59 71 88 96 103 110 113 40 60 8 12 54 67 80 91 98 106 114 122 35 42 48 55 61 64 67 71 37 44 51 59 64 71 78 84 20 80 4 16 5 5 5 5 5 5 6 6 5 5 5 5 5 5 5 5 6 6 7 8 10 11 12 13 0 100 0 20 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Averages Concentration (Water/hydrogen peroxide) Time (seconds) Average Volume of Oxygen produced (cm�) Amount of H�O� (%) Amount of H�O (%) Amount of H�O� (ml) Amount of H�O (ml) 30 60 90 120 150 180 210 240 100 0 20 0 101 134 152 163 173 182 187 193 80 20 16 4 84 91 95 102 122 141 162 179 60 40 12 8 54 66 81 95 106 113 120 125 40 60 8 12 42 51 60 68 74 80 86 92 20 80 4 16 5 5 6 6 7 7 8 8 0 100 0 20 0 0 0 0 0 0 0 0 Conclusion: Looking at our graphs ...read more.

Conclusion

This was confirmed as we compared our error bars and averages to the other class members showing that our results were correct and our error bars were small. We followed our method properly keeping to the health and safety aspects at all times and staying alert writing down any notes on the changes of each of the experiments. This allowed us to keep a record of what happened at the time when it happened so were not working by memory but on valuable and reliable notes. After looking at the error bars for the experiment, we can see that the experiment we have done for the 20% concentration was extremely reliable. However, as the experiments went on, the results became further apart, which suggests that the experiments we performed on the other concentrations were not as reliable as they could have been. These could have been caused by the different factors which were talked about earlier. Despite the size of some of the error bars, the general trend still follows the theory. If we had more time, we would have repeated the experiments again, so we would not have any outliers. This would have made the results more reliable. The conclusion is based around the collision theory; this is most reliable theory to explain the test that I have completed. This theory makes my conclusion very reliable as most scientists agree with the facts produced alongside the highly tested collision theory and as I have explained in my conclusion it is the theory of the collation between the molecules producing the reaction. Working completely in our group of three we managed to complete the task to our full potential, this allowed us to see the changes occurring as we changed the different concentrations of the ph buffer and of the concentration of the catalyse source (which I would change if I were to do it again). ...read more.

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