• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Enzyme Reaction - Catalase. Aim: To test the reaction of the enzyme catalase in various ph levels.

Extracts from this document...

Introduction

Title: Enzyme Reaction - Catalase Date: February 14, 2012 Aim: To test the reaction of the enzyme catalase in various ph levels. Materials: liver potato, knife, H2O2, HCL, NaOH, four test-tubes, forceps. Method: Four pieces of liver were cut in 1cm square. Four test-tubes containing about 2cm^3 of H2O2 were set up. A piece of liver was placed in one test-tube. Another piece of liver was crushed and added to another tube. 1 cm^3 of HCL was added to the third test-tube and then another piece of liver was also added. 1cm^3 of NaOH was added to the last test-tube followed by another piece of liver. This was repeated except potato was instead used in the place of the liver. ...read more.

Middle

POTATO Bubbles quickly began to form and the test-tube became warm. Bubbles were formed and the bottom of the test-tube became warm. Few bubbles were released from the potato The potato turned a light yellow color and few bubbles were released. Conclusion: This enzyme's performance is influenced by pH. Catalase enzyme works optimally in neutral condition. It is proven by the amount of bubbles produced in the reactions. The more bubbles produced the faster the enzyme works and vice versa, because one of the enzymes function is to catalase fasten the reaction rate. Addition of acids, base, or extreme heat will break the enzyme, specifically oxygen will not be able to be produced. Discussion: Enzymes are proteins that catalase biochemical reaction. ...read more.

Conclusion

The enzyme has an active site that helps it to recognize its substrate in a very specific way. Just like a key only fits into a specific substrate. This is called the lock and key theory. All enzymes have a specific temperature that they work that perfectly at, that makes them collide more frequently. If they got hotter than this temperature, which is usually about 45�C the enzyme dentures and stops working. Catalase is an enzyme found in both foods tested. It is used for removing hydrogen peroxide form the cells. Catalase speeds up the decomposition of hydrogen peroxide because of the shape of the hydrogen peroxide molecule. This type of reaction where a molecule is broken down into smaller pieces is called an anabolic reaction. ` In the experiment the liver showed more reaction because it has a greater metabolism more catalase is needed to remove this toxic compound. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a star student thought of this essay

3 star(s)

Response to the question

This experiment is structured well, but it is missing a hypothesis. A hypothesis shows the examiner you are applying scientific knowledge throughout. Something along the lines of: "As the pH levels increase, it is expected that there will be fewer ...

Read full review

Response to the question

This experiment is structured well, but it is missing a hypothesis. A hypothesis shows the examiner you are applying scientific knowledge throughout. Something along the lines of: "As the pH levels increase, it is expected that there will be fewer bubbles formed during the experiment". This then allows you to draw upon it within your conclusion and explain it fully in the discussion section. Examiners like to safety taken into consideration, and a more thorough method would've allowed this.

Level of analysis

In scientific experiments, clarity and accuracy is key. Using phrases such as "four test tubes containing about" shows to the examiner that you don't fully understand the significance of a fair test. The results table is good, giving only qualitative responses of what can be seen. There does need to be a column heading, however, above liver and potato such as "Tissue Type". The discussion at the end is average. There is a sound knowledge of enzymes and the factors which affect their rates of reaction, but there isn't a thorough discussion of pH levels. When the experiment is specific, you need to spend time discussing the factor in detail. I would've liked to have seen some discussion of how pH level breaks the bonds which make the active site specific (at GCSE it isn't necessary to discuss which bonds break) and then an enzyme-substrate complex cannot form.

Quality of writing

Examiners are always picky with coursework pieces. In an exam you may get away with "ph levels" but you may be penalised otherwise for not writing it as "pH levels". There are times where the grammar is wrong. Although it isn't as important in scientific pieces of work, there will always be marks for Quality of Written Communication (QWC) so it is key these errors are picked up in a drafting process.


Did you find this review helpful? Join our team of reviewers and help other students learn

Reviewed by groat 13/07/2012

Read less
Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Plan: The effect of the end product, phosphate, on the enzyme phosphatase

    5 star(s)

    Therefore, the absorbance decreased as their corresponding moles of sodium phosphate increased. If I add more sodium phosphate, I believe the rate of reaction will be 0 as all the enzymes have been inhibited. Evaluation Variability of results Overall all the results coincide with the expected outcome, as the absorbance decreased when moles of sodium phosphate increased.

  2. Marked by a teacher

    The Effect of pH on Catalase

    4 star(s)

    Store the buffers in clearly labelled bottles. Glassware: The syringe plunger slides out easily, therefore secure it to the main part of the gas syringe, but also make sure that this will not restrict any movement of the plunger. Take care of other glassware, keeping them away from the edge

  1. Marked by a teacher

    Biology Coursework Enzymes

    4 star(s)

    * Fourthly I will measure 2 ml of 1% starch into my cleansed 10ml-measuring cylinder and then pour the starch into another one of my boiling tubes. * I will then measure 8 ml of water using my washed

  2. Influence of pH on the activity of potato Catalase

    Readings were taken to the nearest second when measuring the time taken for the manometer to rise 3cm. this was a small enough unit of measurement as any small would start to rely on the spend and accuracy of our own measuring.

  1. Action of the enzyme catalase.

    5 cubes (1cm3) of liver and potato is cut. III. The followings are added to test tubes 1 to 8. 1. a sprinkle of sand 2. 2 cm3 of MnO2 3. one cube of liver 4. tube 3. is repeated, the temperature of the H2O2 is measure before and after the addition of the liver 5.

  2. Investigating an enzyme-catalyzed reaction

    If the temperature is above 40�C the enzyme can become denatured which again leads to the shape of the active site changing. The temperature at which enzymes work best at 37�C. Surface Area- If there is a larger surface area more of the enzyme is in contact with the substrate, leading to an increased reaction rate.

  1. Structures and functions in living organisms. Revision Notes

    of cells to include the effects of surface area to volume ratio, temperature and concentration gradient Surface area to volume ratio: A high surface area to volume ratio leads to a higher rate of movement in and out of cells because the membrane which particles can cross will be larger

  2. Aim To explore the reaction of enzymes throughout various pH levels.

    Whilst allowing the solution to cool, prepare the pH buffers into test tubes. 7. Mix 1ml of Amylase with 10mls of pH buffer, leave to stand for 5 minutes. 8. Using a pipette, place a single drop of Iodine into each spotting tile. 9.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work