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Enzyme Reaction - Catalase. Aim: To test the reaction of the enzyme catalase in various ph levels.

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  • Essay length: 578 words
  • Submitted: 13/05/2012
  • Reviewed by: (?) groat
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GCSE Life Processes & Cells

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The first 200 words of this essay...

Title: Enzyme Reaction - Catalase

Date: February 14, 2012

Aim: To test the reaction of the enzyme catalase in various ph levels.

Materials: liver potato, knife, H2O2, HCL, NaOH, four test-tubes, forceps.

Method:

Four pieces of liver were cut in 1cm square. Four test-tubes containing about 2cm^3 of H2O2 were set up. A piece of liver was placed in one test-tube. Another piece of liver was crushed and added to another tube. 1 cm^3 of HCL was added to the third test-tube and then another piece of liver was also added. 1cm^3 of NaOH was added to the last test-tube followed by another piece of liver. This was repeated except potato was instead used in the place of the liver. A table was drawn to record the reaction observed.

Observation:

Table showing enzyme reaction with various solutions on different surface areas.

EFFECTS OF WHOLE TISSUE WITH H2O2

EFFECTS OF CRUSHED TISSUE WITH H2O2

EFFECTS OF WHOLE WITH H2O2 AND HCL

EFFECTS OF WHOLE TISSUE WITH H2O2 AND NaOH

LIVER

Bubbles began to rapidly form and the test-tube became warm.

Bubbles were formed

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Review of essay

Reviewed by: groat

Rating: 3 star(s)

Response to the question

This experiment is structured well, but it is missing a hypothesis. A hypothesis shows the examiner you are applying scientific knowledge throughout. Something along the lines of: "As the pH levels increase, it is expected that there will be fewer bubbles formed during the experiment". This then allows you to draw upon it within your conclusion and explain it fully in the discussion section. Examiners like to safety taken into consideration, and a more thorough method would've allowed this.

Level of analysis

In scientific experiments, clarity and accuracy is key. Using phrases such as "four test tubes containing about" shows to the examiner that you don't fully understand the significance of a fair test. The results table is good, giving only qualitative responses of what can be seen. There does need to be a column heading, however, above liver and potato such as "Tissue Type". The discussion at the end is average. There is a sound knowledge of enzymes and the factors which affect their rates of reaction, but there isn't a thorough discussion of pH levels. When the experiment is specific, you need to spend time discussing the factor in detail. I would've liked to have seen some discussion of how pH level breaks the bonds which make the active site specific (at GCSE it isn't necessary to discuss which bonds break) and then an enzyme-substrate complex cannot form.

Quality of writing

Examiners are always picky with coursework pieces. In an exam you may get away with "ph levels" but you may be penalised otherwise for not writing it as "pH levels". There are times where the grammar is wrong. Although it isn't as important in scientific pieces of work, there will always be marks for Quality of Written Communication (QWC) so it is key these errors are picked up in a drafting process.

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