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Enzyme Reactions

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Amish Gandhi 24.9.2001 Enzyme Reactions Introduction In lessons I am researching into enzymes and their functions, also what affects them. The basis of this investigation is to find out what affects the rate of reaction while using enzymes. In the experiment I am testing whether the concentration of the acid (hydrogen peroxide) affects the amount of reaction and if any in what way. I will conduct this experiment by testing the differences in the amount of reaction in a minute. The reaction will be made by reacting catalyse (found in potatoes, which I'll cut up), and hydrogen peroxide. To change the concentration I will dilute the hydrogen peroxide in steps using water. I will measure the amount of gas given off (measured in bubbles) in a minute. Background Information The chemical equation for this experiment is: Hydrogen peroxide catalyse water + oxygen Enzymes Enzymes are basically catalyst with their own uniqueness. Unlike other catalysts they have a very delicate structure, this makes them venerable to become denatured if exposed to high temperatures. Denatured is when the enzymes stops functioning, this does not mean it is dead as it was never alive. Enzymes are classified into categories, these are; hydrolytic, oxidizing, and reducing enzymes. ...read more.


This is only after the products have been released though. However enzymes cannot penetrate living cells. They have to be dead before the enzymes can pass through. This is because that the cell membrane would not let through the enzyme. When the cell is dead the cell membrane becomes permeable and the enzyme can pass. My Plan For my experiment I will use a measuring test tube so I can change how diluted the hydrogen peroxide is. I will need a test tube to put my potato and hydrogen peroxide in, the test tube will need a bung and a tube for the gas to travel through to a measuring test tube to measure the amount of gas made. I will keep my experiment a fair test by making sure my amount of water diluting the hydrogen peroxide is exactly what it is meant to be (the amount of hydrogen peroxide will vary as well to always make 10 cubic centimetres). Then I will need to make sure the potato pieces I use will have the same surface area and that all sides have been cut to perfection so the catalyse can leave. Another factor that could affect the reaction is the temperature, so I will have to make sure that the experiments will always be held at room temperature. ...read more.


I repeated Number 1, which first came to 8 bubbles. At the start it seemed that Number 2 was wrong with 6 as it was less than 8 (number 1) and also that Number 4 had 9 bubbles. However I did all the takings before I jumped to the conclusion that which ones were wrong and that way I found out which takings were inaccurate. Evaluation I think that my experiment was fairly accurate, but that errors were probably made, while measuring out small amounts of Hydrogen peroxide and Water. Perhaps if I used a larger quantity of the two if I had made any mistakes it would not be as significant (I would probably make my potato piece bigger as well). I would do that if I could do the experiment again, but also I would find a method a chopping my potatoes so that they are more accurate. Other flaws with my investigation include that I couldn't completely control the temperature; this was due to the fact that other experiments were happening and also there were many other people in the classroom, which gave off body heat. Also heat was made from people move (kinetic heat). I believe that my experiment was reliable enough to base an explanation, on how concentration of the substrate affects a reaction. However if I did do it again I would expect it to be considerably better using my suggestions. ...read more.

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