The Activity Of An Enzyme At Different Temperatures
Aim:
The aim of this experiment was to test the rate of enzyme activity between amylase and starch at different temperatures and to find the ideal temperature for reactions to take place the quickest in.
Apparatus:
The apparatus used includes:
- Stop Watch
- Count-Down Timer
- Starch
- Amylase
- Paper Pad To Record Results
- Iodine
- Thermometer
- Pipette
- Dropper
- Beaker
- Kettle
- Ice Cubes
- Water
Method:
- We placed two drops of iodine each onto the 12 depressions of the dimple tray.
- Using a syringe, we placed 5cm3 of starch solution into one test tube.
- Using another syringe, we placed 5cm3 of amylase solution into another test tube.
- We had to half fill a beaker with water at room temperature. We either added some boiling water from the kettle or some ice cubes to get the desired temperature.
- We then put both the test tubes containing amylase and starch into the beaker of water for 5 minutes.
- After the wait, we poured the test tube containing amylase solution into the one that had the starch solution. The test tube with the mixture had to be shaken so it would mix. The test tube was still kept in the water bath. The stop-watch was started as soon as the test tube was back in the water. The empty test tube was put aside.
