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Enzymes experiment. Do enzymes work faster or slower when the temperature is high?

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Aim Do enzymes work faster or slower when the temperature is high? I am investigating if changing the temperature makes the enzyme takes longer to break down the protein strip by timing how long it takes to break down. The reason to why I am doing this is because I need to find out if the reaction time of the enzymes is affected by different temperatures. Range My range will be going up 10�C each time I will be starting with 30�C then it will be going up to 40�C, 50�C, 60�C, and the final temperature would be 70�C. If I feel that it's taking too long I will be decreasing the temperature by about 6�C for each time. Variables I won't be changing anything in my experiment apart from the temperatures I will be keeping the protein strip the same size and it won't be changed however I'm only going to change the temperature that the protease is also I'm going to be repeating the test for 3 times on each to make sure that the test is fair then I'm going to have an average between the three different times I'm going to be converting the minutes to seconds. I will be using 6ml of protease and the protein strip that I have measured is going to be around 3.5cm. The concentration of the protease that I'm going to use is 2%. Apparatus I will use a kettle the use of the kettle is that it will heat up the water that I'm going to put my protease and protein strip will sit in the test tube. I chose a kettle because I felt that it was the easiest way to heat up the water, it was also quick as well. I also thought it was easier to get the temperature I wanted because it will be hot and it does take much shorter period of time to get the water hot also one more advantage was that if it was too hot then I could add cold water if needed. ...read more.


Therefore this would be the only change that I am going to have I my real test so therefore I would have more reliable results. The Main Test I looked at my method I only changed my water bath to kettle and what I done was I used the 5 different temperatures and to make it a fair test I repeated it 3 times and I came up with an average the way that I made the average is I converted the minutes to seconds then added the 3 test that I done together then divided it by 3. These are the results that I got from the test: Time Taken to Clear Temperature (Degrees) Try 1 (Minutes) (Seconds) Try 2 (Minutes) (Seconds) Try 3 (Minutes) (Seconds) Average (Minutes) (Seconds) 30�C 2.52 (m) 172 (s) 2.21 (m) 141 (s) 2.14 (m) 134 (s) 2.29 (m) 149 (s) 40�C 1.20 (m) 80 (s) 1.03 (m) 63 (s) 1.16 (m) 76 (s) 1.13 (m) 73 (s) 50�C 4.32 (m) 272 (s) 4.01 (m) 241 (s) 4.12 (m) 252 (s) 4.15 (m) 255 (s) 60�C 4.12 (m) 252 (s) 4.55 (m) 295 (s) 4.03 (m) 243 (s) 4.23 (m) 263 (s) 70 �C 5.25 (m) 325 (s) 4.57 (m) 297 (s) 5.12 (m) 312 (s) 5.11 (m) 311 (s) Data To get accurate results I repeated this test three times to make it fair test. This allows me to have more data to work with and it can be more accurate. It also means that I will know if I have gone wrong in some aspects of my test. I had a fairly acceptable and medium sized range; it covered the cool temperatures and the high temperatures as well as our normal body temperature. However, after the first attempt of my experiment, I found that I could not get the protease to reach the right temperature, which at the time was 20 degrees. ...read more.


However overall when I looked at my other temperatures I was able to see that the error bars was small and also my lone of best fit was reliable and I know that I can justify the reasons that I had outliners. I think that my data is reliable the reason for this is because when I have reflected back onto my data I actually felt that it was reliable. The main reason as to why I thought this is because when looked at my data I had seen that I had 2 outliers and 3 that went into the line of best fit. This shows that my results were accurate however fit was 3 outliers this would mean that it wasn't accurate and reliable. Also when I looked on my data I saw that there were 2 results that had a big error bar and 3 was accurate this also means that if I had 3 bad error bars then this would mean that my results hasn't been accurate. I think that my range was very accurate the reason for this is because it was about 10 the reason that I think this was good is because when you use a range of 10 you can then see the difference much more easier. Also if I had something wrong then I would have been able to change it much easier. I am very confident with my data the reason for this is because whilst I was doing this I had found out that my results was effective and I knew every way that I can improve and I have justified this in many ways. I think that my technique was good and I didn't really find it very hard to be honest. Also I think that I could have made it much more accurate the reason for this is because I know what to do for next time and I know every way that I need to make sure that I get it right. ?? ?? ?? ?? Elgun Basyurt 11A Enzyme Coursework Science ...read more.

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