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Experiment- Do people have the same amount of salivary amylase?

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Introduction

Salivary Amylase Lab Scotty Keller Background- Amylase is secreted by the pancreas, small intestine wall and salivary glands to aid digestion by the hydrolysis of starch into maltose. Also, IKI2 is a starch indicator meaning it can be used to deduce the concentration of a starch solution. The higher the concentration of starch; the darker the color of the combination. Question- Do people have the same amount of salivary amylase? Hypothesis- Not all human beings have the same amount of salivary amylase. This can be shown by the fact that, according to the USDA's National Nutrient Database for Standard Reference, different people digest food at different rates and, according to Dr. Stephen Gangemi of DrGangemi.com, some people are incapable of digesting starch. This is known as starch intolerance. Independent Variable- How much amylase is contained in the saliva? Dependent Variable- How quickly will my saliva hydrolyses starch into maltose? Controls- 1. The concentration of the initial starch solution. 2. The amount of the starch/distilled H2O solution used. ...read more.

Middle

This dilution to 10% the previous concentration is necessary to lessen the amount of amylase that hydrolyses the starch in order to slow the reaction down far enough that it can be accurately observed. 4. Use the pipette to add 1 ml of the diluted saliva to the test tube. Rinse pipette with distilled water. Empty and rinse graduated cylinder with distilled water. 5. Use the pipette to add 7 ml of the starch to the graduated cylinder. Rinse the pipette with distilled water. 6. Pour the prepared 7 ml of the starch solution into the test tube and stir with the stirring rod. Begin timer. Rinse pipette with distilled water. Wait 15 minutes. 7. After 15 minutes, combine 6 drops of the saliva/starch, using the pipette, and 10 drops of IKI2, using the included dropper, in the well plate. This will result in a yellow liquid with a darkness based on the amount of starch left over from the hydrolysis. 8. Compare the resulting colored well to the wells of the previously prepared color guide to deduce the concentration of starch in your solution. ...read more.

Conclusion

However, the conclusions of this lab further support the evidence provided by the USDA's National Nutrient Database for Standard Reference and Dr. Stephen Gangemi of DrGangemi.com that proves that people do not have the same amount of salivary amylase. The procedure I followed could have been improved in several aspects. First, there were bubbles in the saliva that made measuring it out difficult. Though it would not have been practical, had we been given enough time for our lab, we could have tried to wait for the saliva to settle. Also the clock that was used to time the hydrolysis was not very accurate. The use of a simple stopwatch could have fixed this problem. The use of IKI2 as an indicator was extremely inaccurate. Perhaps come type of starch probe would have been able to more accurately indicate the concentration of starch. To perfect the lab we could have used some kind of amylase probe and directly measure the amount amylase in the saliva. Though there were many shortcomings to this investigation it was clear in showing that not all people have the same amount of amylase. ...read more.

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