• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Experiment to determine the effect of temperature on the permeability of a cell membrane .

Extracts from this document...


Experiment to determine the effect of temperature on the permeability of a cell membrane . Implementing Safety. To carry out the experiment fairly it was compulsory to ensure that the beetroot was diced into precisely one centimetre cubed segments. To obtain these segments we had to bore into the beetroot to obtain a cylinder shape with a constant diameter, to be sure that the borer couldn't harm us it was necessary to hold the beetroot securely making sure that your hand wasn't in the path of the borer, it was also important to screw the borer away from your body. The long cylinder had to then be dice using a sharp scalpel that cased less damage to the membranes, however it may have been dangerous if a person did not apply their full concentration when using. Users must be careful concerning how the scalpel was held and used. Water baths were used to determine the effect of temperature on the permeability of the membrane; the water baths were set at varying temperature up to 61 degrees C the higher temperature may have been painful if splashed on the skin therefore it was essential to take care when placing the test tubes in to the bathes. ...read more.


The graphs I have drawn shows some conflicting results between the results of test tube one and test tube two. Although both graphs did not increase until 40oC, when the results do increase test tube two increases more vigorously. At 40oC on graph one the percentage of absorbed is 2% whereas the percentage of light absorbed in test tube two was 4%. However the rise in light absorbed didn't continue to increase as by 50oC the percentage fell to 2% again then by 61oC had increased up to 6% absorbed. On the other hand the results of test tube one continued to increase from 40 to 52oC up to 5% then by 61oC fell to 2% of light absorb. However the graph that showed the average % of light absorbed had a positive correlation through out, although it does level out at some points. For example from 2 to 31oC the percentage stays at 0.5 percent. The final graph compares all three sets of results that were obtained and shows the differences between the results of test tube one and two. Further calculations Concluding I conclude that increasing the temperature increases the permeability of the membrane, allowing more pigment to be released into the distilled water. ...read more.


The Individuality of the beetroot may have caused our results to be different from other peoples in the class. Just like humans have different amounts of pigment in their skin so do beetroot, our specific beetroot may have contained less pigment. Therefore causing the % of light absorbed to be lower. Effect of limitations I don't believe that these limitations will have a great affect on the over all results of our experiment, due to the fact that the results we have obtained from the original and repeat were very similar in most cases. Although there are some anomalous results, this does not seem to have affected the average as it still increases with the increase of the temperature. Which would have been expected due to the temperature increasing the permeability of the beetroot cell membrane allowing more pigment to pass through. The wider range of temperatures would have enabled us to pin point the temperature at which the cell membrane becomes denatured, which would of made the experiment more accurate, however this didn't affect our experiment a lot because we were investigating the effect of temperature on a cell membrane which can be clearly seen by our results. The reliability of our apparatus didn't affect us either because the result increase as we would of expected. The accuracy of our results could be tested against other groups or against other repeats. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Green Plants as Organisms section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Green Plants as Organisms essays

  1. Marked by a teacher

    How does temperature affect the permeability of a cell membrane in a beetroot.

    4 star(s)

    Amount of water The amount of water the beetroot is placed in will affect the end result of % of light transmitted. I will be place the beetroot in 10ml of water. I have decided on this amount from my preliminary work in which I used 25ml which I found to be considerably too much water.

  2. Determine the water potential of potato tuber cell with the varying affect of solute ...

    However in this experiment we ourselves did not add any external pressure. This is why each cylinder has the same pressure so we count it as zero. This means that when you look at the graph and find the solute potential you have automatically found the water potential since there is no pressure potential.

  1. An investigation in to the effect of temperature on the release of pigment from ...

    I feel this will help me get a more accurate result for each temperature range. This is because, with only one reading or two readings, the readings could have been achieved or affected by the external factor, and not achieved solely due to my IV - the increase of temperature.

  2. the effect of temperature on membrane permeability

    Work quickly to allay cooling of the solution. 11. Using a pipette, fill a cuvette 3/4 full of beetroot pigment solution. Be careful to handle only the frosted or rigid sides of the cuvette to prevent 'polluting' the clear sides 12.

  1. How Temperature Effects the Movement of Pigment Through Cell Membranes

    structure and as the protein function is dependent upon this, it becomes useless. This malformation in the protein also increases the fluidity of the membrane as the protein is rejected by the cell when denatured and so creates gaps in the membrane structure allowing anthocyanin to diffuse out.

  2. Investigating the effect of temperature on permeability of membranes

    The effect of the high temperatures can cause physical damage to the cell wall or cytoplasm, for example by expansion and rupture of cells. In this case the cell sap would subsequently leek out. I predict that temperatures of above 50 degrees Celsius would have the most effect on the membrane and most anthocyanin would be escape.

  1. An investigation to find out how temperature affects membrane permeability.

    Therefore a higher temperature will denature the proteins and diffusion will be able to take place easily due to the proteins being unable to manage what enters or leaves the cell. The optimum temperature of the beetroots normal working conditions should be around 40 �C as the optimum temperature for

  2. An experiment to test the effect of different temperatures on the permeability of cell ...

    * The cell membrane is often seen as a fluid mosaic model, and it is mosaic due to all the different proteins and cholesterol among the phospholipids. In normal condition the dye would not be able to pass through this complex membrane layer, but when temperature rises and gets above

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work