Experiment to determine the effect of temperature on the permeability of a cell membrane .
Implementing
Safety.
To carry out the experiment fairly it was compulsory to ensure that the beetroot was diced into precisely one centimetre cubed segments. To obtain these segments we had to bore into the beetroot to obtain a cylinder shape with a constant diameter, to be sure that the borer couldn't harm us it was necessary to hold the beetroot securely making sure that your hand wasn't in the path of the borer, it was also important to screw the borer away from your body. The long cylinder had to then be dice using a sharp scalpel that cased less damage to the membranes, however it may have been dangerous if a person did not apply their full concentration when using. Users must be careful concerning how the scalpel was held and used.
Water baths were used to determine the effect of temperature on the permeability of the membrane; the water baths were set at varying temperature up to 61 degrees C the higher temperature may have been painful if splashed on the skin therefore it was essential to take care when placing the test tubes in to the bathes.
There were very little risks of serious injury, any accidents which may of occurred would have been down to lack of concentration on their parts.
Results-
Temperature oc
Test tube 1 % of light absorbed
Test tube 2 % of light absorbed
Average % of light absorbed
2 (Ice)
1
0
0.5
31
1
0
0.5
40
2
4
3
52
5
1
3
61
2
6
4
Precautions taken.
To ensure that the experiment was as reliable as possible, there were many precautions taken, Such as controlling the many variables. It was important to monitor that the water baths were a constant temperature; otherwise the result for a certain temperature wouldn't have been accurate. Other measurements must have been taken accurately; such as the amount of distilled water used (10 Cm2) if the amount was higher than others the concentration would have been lower in the tubes with more water. The segments of beetroot were measured and cut accurately otherwise the larger cubes would contain more pigment meaning the concentration would be higher in tubes containing larger cubes.
Implementing
Safety.
To carry out the experiment fairly it was compulsory to ensure that the beetroot was diced into precisely one centimetre cubed segments. To obtain these segments we had to bore into the beetroot to obtain a cylinder shape with a constant diameter, to be sure that the borer couldn't harm us it was necessary to hold the beetroot securely making sure that your hand wasn't in the path of the borer, it was also important to screw the borer away from your body. The long cylinder had to then be dice using a sharp scalpel that cased less damage to the membranes, however it may have been dangerous if a person did not apply their full concentration when using. Users must be careful concerning how the scalpel was held and used.
Water baths were used to determine the effect of temperature on the permeability of the membrane; the water baths were set at varying temperature up to 61 degrees C the higher temperature may have been painful if splashed on the skin therefore it was essential to take care when placing the test tubes in to the bathes.
There were very little risks of serious injury, any accidents which may of occurred would have been down to lack of concentration on their parts.
Results-
Temperature oc
Test tube 1 % of light absorbed
Test tube 2 % of light absorbed
Average % of light absorbed
2 (Ice)
1
0
0.5
31
1
0
0.5
40
2
4
3
52
5
1
3
61
2
6
4
Precautions taken.
To ensure that the experiment was as reliable as possible, there were many precautions taken, Such as controlling the many variables. It was important to monitor that the water baths were a constant temperature; otherwise the result for a certain temperature wouldn't have been accurate. Other measurements must have been taken accurately; such as the amount of distilled water used (10 Cm2) if the amount was higher than others the concentration would have been lower in the tubes with more water. The segments of beetroot were measured and cut accurately otherwise the larger cubes would contain more pigment meaning the concentration would be higher in tubes containing larger cubes.
