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Experiment to investigate reaction.

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Introduction

Experiment to investigate reaction Catalase Aim: I want to find out what will speed up the reaction H2O2´┐ŻO2+2H20. Factors: The factors of my experiment which could affect what happens in my investigation are; Temperature, amount of potato catalase, amount of concentration of hydrogen peroxide, time, amount of fairy liquid used and sunlight or darkness. The investigation: In order to make sure it is a fair test I am only going to change one factor which is the amount of potato catalase used. I will keep all the other factors the same in each test tube. My prediction: In my investigation I think the rate of reaction will increase the more potato catalase used. I think this because of my scientific knowledge of how an enzyme works and how reactions speed up. In order to explain this to you I will use the lock and key theory and the collision theory as examples. The lock and key theory is when sufficient subtrate locks into the active site then splits into two separate compounds. E.g. potato catalase splits hydrogen peroxide into two separate compounds. The collision theory is when particles have sufficient energy to collide together and react, the minimum amount of energy needed is known as the activation energy. ...read more.

Middle

I will put the test tubes in a test tube rack in order to stop them being knocked over and broken. I will use the equipment carefully and clear all used equipment out of the way to prevent them getting in the way. I will roll up my sleeves and remove my chain to stop it getting in the way of our experiment. Here are my results: Time in minutes 3g of potato catalase 2g of potato catalase 1.5g of potato catalase 1g of potato catalase 0.5g of potato catalase 1 17cms of froth 15cms of froth 10cms of froth 7cms of froth 5cms of froth 2 23cms of froth 20cms of froth 15cms of froth 10cms of froth 7cms of froth 3 30cms of froth 27cms of froth 20cms of froth 15cms of froth 10cms of froth 4 44cms of froth 40cms of froth 27 cms of froth 20 cms of froth 15 cms of froth 5 50 cms of froth 44 cms of froth 30 cms of froth 25 cms of froth 16 cms of froth 6 57 cms of froth 47 cms of froth 36 cms of froth 30 cms of froth 18 cms of froth 7 63 cms of froth 51 cms of froth 46 cms of froth 33 cms of froth 20 cms of froth ...read more.

Conclusion

If I had to use my results as evidence they show that the more potato catalase used the faster the rate of reaction. The reason I think this happened is because of the lock and key theory and the collision theory where the subtrate fits and locks into an active site then splits into two separate compounds (lock and key theory). The collision theory is when particles collide (when they have enough activation energy) to cause a reaction. In order to make my investigation better I should have staggered the results at the start time so when I measured the foam I had enough time to measure each one rather than rush through them all at the same time so that only the 1st would be on time but by the time you get to the 5th you would be at least 10 seconds into the next minute so the 5th had an unfair advantage as it had an extra 10 seconds. I f I were to stager the start time I could do them 5 seconds apart so I have enough time to measure each one. To get further evidence I could use different volumes of hydrogen peroxide and the same size chip to see if that affects the rate of reaction. I could use more fairy liquid or put them all at different temperatures to see if that varies the rates of reaction. ...read more.

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