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Experiment to investigate the effect of temperature on respiration in yeast.

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Experiment to investigate the effect of temperature on respiration in yeast. In my experiment I used the following I used the following apparatus: * Test tube * Delivery tube * Thermometer * Beaker (300ml) * Beaker (100ml) * Yeast * Glucose * Tripod * Bunsen burner * Heating mat * Measuring cylinder * Stopwatch In my experiment I am investigating how temperature effects the respiration in yeast. Method In my experiment I first of all gather the above apparatus. I will fill the three hundred mills beaker with water and then place my test tube inside of it, the test tube will contain, so many parts glucose and so many parts yeast, but both parts have to add to twenty-five mills. (Each time so that it will be a fair test). The test tube will have a rubber bung on the top of it, which is attached to a delivery tube, and the other end of the delivery tube was in a smaller beaker, which is filled with water so that I could count the bubbles. Once this was set up, I heated the large beaker; the water heated, which heated the yeast then gave off gas through the delivery tube, which created bubbles in the smaller beakers water. ...read more.


31 25 34 24 35 27 37 36 38 38 41 42 43 44 44 45 48 46 52 48 53 40 55 26 59 18 61 3 63 0 65 0 68 0 69 1 70 0 75 0 80 0 86 1 90 0 Temperature Bubbles/minute 19 6 21 9 23 10 24 12 26 14 28 25 31 24 34 27 35 36 37 38 38 42 41 44 43 45 44 46 48 48 52 25 53 18 55 3 59 1 61 0 63 0 64 0 65 0 68 1 69 0 70 0 75 0 80 0 86 0 90 0 The things that may make my experiment inaccurate could be: * Time * Temperature * Measurement * Same apparatus. In my experiment I didn't make any mistake to my knowledge, I put my stool under my table, wore goggles, and cleared my desk so that that I would be safe from any chemicals that may spill. The measurements I used added up to twenty-five for example: five mills glucose and 20 mills yeast. (These changed each time) The average amount of bubbles= 532 % 29 = 18. 3 bubbles (1.dp) The first set of results told me that: between o% and 18% there wasn't a change in the respiration of yeast and ...read more.


My results did help me solve my problem because the temperature did effect the respiration in yeast (as I stated above). I think my results are fairly reliable but not as reliable, as If I would have done them more than twice. If I was going to do my experiment and whole investigation again I would the only thing I would change would be more results. So I would have a firmer conclusion. Also because my formula added up to 25 mills I think I would increase that to about 50 mills and see if this made any difference. If I were going to increase the concentration to 50 mills I think my prediction would be that instead of the peak temperature being around 62% I think I t would be around 100%. I thought this because it would take longer for the yeast to respirate. There were a number of things that were inaccurate in my experiment the first one being the temperature, I wasn't sure if I was supposed to start the stopwatch then record the bubbles for 30 seconds or time 30 seconds then record the bubbles. Looking back now on my experiment I see that I should of changed my water every time, i.e.) once I have recorded the bubbles for 10 degrees then change the water and then heat it too 20 degrees and record it. ...read more.

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