• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Experiment to investigate the relationship between the number of stomatal pores on the upper and the lower surfacesof the leaves of a mesophyte plant and the rate of transpiration from those surfaces.

Extracts from this document...

Introduction

Experiment to investigate the relationship between the number of stomatal pores on the upper and the lower surfacesof the leaves of a mesophyte plant and the rate of transpiration from those surfaces. The aim of the experiment The aim of the experiment is to investigate how the number of stomatal pores is related to the rate at which water is lost from the leaves. A mesophyte plant is chosen and the comparison is between the upper and the lower surfaces of its leaves. Experimental hypothesis Taking into account the relative background scientific Information, it is expected to be proven that the rate of transpiration from a leaf of a plant is proportional to the number of stomatal pores on the surface of that leaf. Null hypothesis Negative results would be to establish that the transpiration rate is inversely proportional to the number of stomatal pores or is not affected by it whatsoever. Introduction Water is the universal solvent for a huge amount of chemical substances in all living organisms. Plants require water for many different reasons. It is used to uptake inorganic minerals from the ground, to transport nutrients such as amino acids and carbohydrates along their stems and to control their temperature. ...read more.

Middle

Water is also lost from the lenticles of the stems but in the leaves this takes place to a much greater extent. The rate of transpiration is affected by several environmental factors. The difference of the water potential between the leaves and the surrounding air is very important, hence humidity is one of those factors. Wind increases the rate of transpiration while temperature might have different effects on it. There are also several factors which affect the transpiration rate in an indirect way, by controlling the stomatal aperture. Although there is some evaporation from the cuticle, water, as mentioned above, is mostly lost from the stomatal pores. Therefore light intensity, carbon dioxide/oxygen ratio, water stress conditions and the biological clock effect are all closely related to the rate at which transpiration occurs. Besides the number of stomatal pores itself is proportional to the rate at which water is lost from the leaves, which is exactly what this experiment aims to prove. The experiment consists of two major parts Part 1 : Determination of the rate of transpiration on the upper and the lower surfaces of sample leaves of a mesophyte plant. ...read more.

Conclusion

2cm). Carefully place them in petridishes by means of forceps, to avoid contact with hands. Randomly choose a statistically viable number of leaves on the plant (eg. 10) and number them by attaching small labels on the stems. Insert a petridish into the microwave and heat for approximately 5 minutes. Again, by means of forceps, quickly attach the blue cobalt chloride paper on the leaf by a sellotape piece and start timing. Observe the colour change of the cobalt chloride paper as water evaporating from the leaf turns it pink from blue and for more accuracy, compare with a moist sample. Stop timing as soon as the blue colour is lost. Repeat the procedure for five times on each surface of this leaf, then proceed to another leaf until all ten are examined. Record the results into a table. Using a calculator obtain random coordinates on upper and lower surfaces of the leaves and apply a thin layer of nail polish on those sample areas. Leave to dry for 10 to 15 minutes, then remove the layer by means of forceps and transfer onto microscope slides. Count the number of stomata on a fixed area under light microscope. Record the results into a table. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Green Plants as Organisms section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Green Plants as Organisms essays

  1. Does Leaf Surface Area Affect the Rate of Transpiration in a Plant?

    F 34mm 9.5 10 11 30.5 10.17 Conclusion: From the above results which I obtained from the further above experiment, I can draw the conclusion that my prediction was correct and that the rate of transpiration changes in proportion to the surface area of a leaf.

  2. The investigation is aiming to look at transpiration.

    RESULTS Table {1} to show the rate of transpiration in mesophytes using a range of different conditions. Conditions {Inhibitors} Time {Mins} Initial Volume {cm3} Final Volume {cm3} Average Volume {cm3} Average Volume Per unit Time {cm3min-1} Normal 5 2.3 0.0 3.6 1.2 1.3+1.2/2 = 1.25 1.25/5 = 0.25 Electric fan

  1. Three separate experiments which are to be carried out to investigate a plant's unique ...

    As soon as the bubble gets to the 0cm mark on the ruler, the experiment is ready to start. I have decided that the way I am going to record my results are as follows: I will keep the time the same rather than the distance.

  2. What factors affect the rate of transpiration.

    The capillary tube is small and thin, it allows the bubble to move along faster. The beaker is where the source of water is. The ruler is for measuring the distance travelled by the bubble. The stopwatch is to time the experiment.

  1. Find out where the stomata are located, on the upper or lower epidermis of ...

    One possibility is that opening is achieved by ions being actively transported into the guard cells from neighboring epidermal cells, thereby building up the necessary solute concentration for drawing in water by osmosis. There is evidence that in tobacco leaves potassium ions can be actively pumped into guard cells.

  2. Factors affecting the rate of transpiration

    I selected the one with a woody steam and a number of leaves on it. The leaves on the plant were evenly distributed. I set up the potometer using this plant. I wanted to investigate the effect of wind intensity on the plant, and so I kept temperature, humidity light and the plant the same.

  1. Experiment to Compare Stomata Density in Different Dicotyledonous

    13 Cover slips: to cover the epidermis impression once on the slide to flatten the peel and to make sure it is secure and will not be blown away or damaged. 6. One Microscope with a 15X magnification eye piece: to observe and count any stomata present in the

  2. Conducting an experiment to find out what effect the surface area has on the ...

    I will carry out the experiment at room temperature so this variable can be controlled and reliable results can be obtained. 3) Humidity: Increase in humidity means that air can only take in little water from the plants so transpiration slows down. But at drier day the transpiration is rapid.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work