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Explain the effect that temperature, pH and substrate concentration have on enzyme activity

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M1 ENZYMES ASRA SULTAN explain the effect that temperature, pH and substrate concentration have on enzyme activity Enzymes works at different conditions. There are three different factors that affects the enzyme activity. Temperature is one of the factors that affect the functioning of an enzyme. Effect of temperature: The rate of an enzyme-catalysed reaction increases as the temperature is raised, so as the temperature increases the collision between the enzyme and substrate increases as they both have more kinetic energy and it results in more enzymes-substrate being formed. ...read more.


Effect of pH: Enzymes are also affected by changes in pH. The point where the enzyme works best at is called the optimum pH. Optimum pH value varies from one enzyme to another, pH 2 for pepsin and pH 8 for pancreatic lipase. Extremely high or low pH value mostly results in loss of enzyme activity. Enzymes have a 3D shape which is held together by hydrogen bonds, so if the solution is too acidic or too alkaline it denatures the enzyme. Change in hydrogen ions causes the hydrogen bonds holding the enzyme's shape together to break which causes the enzyme to change ...read more.


Because the enzyme's active site is empty waiting for the substrate to bind. But as there is a low substrate concentration it limits the rate at which the products can be formed. Intermediate substrate concentration: as there are more substrate molecules added the rate of reaction increases and results in more reactions because all the active sites are filled and are being used. High substrate concentration: increasing the substrate concentration increases the reaction rate but when excess substrate molecules are added they are unable to find any free active site because all the active site of the enzyme is occupied. At this point adding more substrate will make no difference and the rate of reaction will not speed up any further. ...read more.

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