• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Explain the effect that temperature, pH and substrate concentration have on enzyme activity

Extracts from this document...


M1 ENZYMES ASRA SULTAN explain the effect that temperature, pH and substrate concentration have on enzyme activity Enzymes works at different conditions. There are three different factors that affects the enzyme activity. Temperature is one of the factors that affect the functioning of an enzyme. Effect of temperature: The rate of an enzyme-catalysed reaction increases as the temperature is raised, so as the temperature increases the collision between the enzyme and substrate increases as they both have more kinetic energy and it results in more enzymes-substrate being formed. ...read more.


Effect of pH: Enzymes are also affected by changes in pH. The point where the enzyme works best at is called the optimum pH. Optimum pH value varies from one enzyme to another, pH 2 for pepsin and pH 8 for pancreatic lipase. Extremely high or low pH value mostly results in loss of enzyme activity. Enzymes have a 3D shape which is held together by hydrogen bonds, so if the solution is too acidic or too alkaline it denatures the enzyme. Change in hydrogen ions causes the hydrogen bonds holding the enzyme's shape together to break which causes the enzyme to change ...read more.


Because the enzyme's active site is empty waiting for the substrate to bind. But as there is a low substrate concentration it limits the rate at which the products can be formed. Intermediate substrate concentration: as there are more substrate molecules added the rate of reaction increases and results in more reactions because all the active sites are filled and are being used. High substrate concentration: increasing the substrate concentration increases the reaction rate but when excess substrate molecules are added they are unable to find any free active site because all the active site of the enzyme is occupied. At this point adding more substrate will make no difference and the rate of reaction will not speed up any further. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Experiment to investigate the effect of Temperature on the enzyme activity of Pectinase

    * Leave reactants together at the same temperature for about 10 minutes; use a thermometer to ensure the correct temperature. Label the beaker to keep it distinguishable from others. * Keep container covered with cling film to prevent oxidation of fruit and evaporation of the enzyme.

  2. Free essay

    Investigation Into the Effect of Substrate Concentration On The Activity Of An Enzyme

    many enzymes. * PH: The PH scale is used to measure how much the acid/alkali the solution contains using litmus paper if the number is below 7 then it is acidic and a number above 7 is alkali and 7 is neutral.

  1. How temperature affects enzyme activity?

    I will look at how temperature affects enzyme activity because it is easier to obtain the correct temperature than it is concentration and pH level. In this experiment every other factor, being pressure, pH and enzyme concentration must be kept the same, so that any changes can only be the result of the temperature change.

  2. The Effect of Temperature on Enzyme Activity.

    This can be done by directly heating the boiling tube that contains the hydrogen peroxide solution and the potato catalase. However, this will lead to very large temperature fluctuations while the catalase is breaking down the hydrogen peroxide (i.e. when the boiling tube is near a heat source, ideally a

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work