Explain the effect that temperature, pH and substrate concentration have on enzyme activity

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explain the effect that temperature, pH and substrate concentration have on enzyme activity

Enzymes works at different conditions. There are three different factors that affects the enzyme activity. Temperature is one of the factors that affect the functioning of an enzyme.

Effect of temperature:

 The rate of an enzyme-catalysed reaction increases as the temperature is raised, so as the temperature increases the collision between the enzyme and substrate increases as they both have more kinetic energy and it results in more enzymes-substrate being formed. In very low temperatures, the collision of molecules is not rapid enough for the enzyme to catalyse the reaction and it doesn't work at its best.

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At very high temperatures the the structure of enzymes is damaged and most of enzymes denature. the enzyme loses it shape, the substrate can no longer fit into the active site and so the reaction rate falls.

Effect of pH:

Enzymes are also affected by changes in pH. The point where the enzyme works best at is called the optimum pH. Optimum pH value varies from one enzyme to another, pH 2 for pepsin and pH 8 for pancreatic lipase.

Extremely high or low pH value mostly results in loss of enzyme ...

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