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Factors affecting the action of catalyse.

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Factors affecting the action of catalyse. Aim- To investigate the rate of which the catalyse in this case the potato breaks down hydrogen peroxide (H2 O2) in proportion to the surface area of a potato exposed to the substrate present. Variables- Temperature Surface area Concentration of Substrate Concentration of Catalyse I have chosen surface area because I think the experiment will be fair to conduct and that it will give the best results. Theory- The theory is that the more of catalyse that is exposed to the substrate then the more the enzyme will be able to react and the more oxygen will be given off to help us record this. Enzymes bind temporarily to one or more of the reactants of the reaction they catalyse. In doing so, they lower the amount of activation energy needed and thus speed up the reaction. Formula equation- * Catalyse. It catalyzes the decomposition of hydrogen peroxide into water and oxygen. 2H2O2 -> 2H2O + O2 Prediction- I predict that the more catalyse surface area exposed then the more rate of reaction will occur because the more catalyse will be exposed therefore the more substrate can be formed into products and the more the enzyme can work. ...read more.


Then fill a tub with water and make sure the water is 40 degrees to achieve optimum temperature to get this temperature use water bath water but do not fill it up to the top but about three quarters of the way up 4. After this put a measuring cylinder that can hold at least 50 ml in the tub and fill it up facing downwards and read the mark the water is on and record 5. Then put the tube in the measuring cylinder with out taking the measuring cylinder out of the tub. 6. Then put the potato pieces in the test tube put the bung on. 11. Then cut the potato that is 2 by one by one in four then half each piece. Then do steps 1-9 7. Then shake the test tube and take down where the water has moved down after 2 minutes and minus it form the start mark to get your result 8. Repeat all these steps again but use 15ml, 20ml, 25ml, 30ml of hydrogen peroxide. 9. Then repeat all of the above 2 more times and record. 10. Then cut the potato that is 2 by one by one in half. Then do steps 2-9 11. Then cut the potato that is 2 by one by one in four. Then do steps 2-9 12. ...read more.


This directly corresponds with my prediction using scientific knowledge. Evaluation- The method I used was fairly safe and it was also accurate. This may have been because I had used good equipment and I heated the water to the right temperature just to get the right temperature for the experiment to work at its best. The accuracy of my results was good because I did not see any clear indication of any anomalous using my tables but although this was the case when I plotted my results on a graph it shown there was one anomalous result it was the point where the potato was showing eight cm squared. This may have been because of these following points: 1. Because I may have cut the potato too much and there was pieces missing. 2. Because there was not enough hydrogen peroxide because I didn't measure it well. 3. Because the temperature was not right so it would be not fair. 4. Or there was not a good part of the potato. I think my method was a good one because I used safety and did a fair test to the best I could. To improve the reliability of the evidence I could have repeated the results again or to monitor the heat all the time and to take results every few seconds instead of every 2 minutes. Abdelaziz Marzoug 10E S.1 ...read more.

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