• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Factors affecting the activity of enzymes

Extracts from this document...

Introduction

Factors affecting the activity of enzymes Aim: to investigate the factors affecting the activity of enzymes. Factors: The factors that could possibly affect the activity of enzymes are: - Temperature: rate of reaction increases as temperature increases. Concentration of enzyme: rate of reaction increases as concentration of enzyme increases. pH: extremes of pH denature enzymes. Surface area: rate of reaction increases as surface area increases. Inhibitors: if there are any impurities, then they will restrict the rate of the reaction. Key Factor: I will be using temperature as a variable, and keeping all other factors constant. Therefore, testing temperature as the key factor. Prediction: I believe, that as the temperature of the starch and amylase increases, as will the rate of the reaction. However, once the optimum temperature is reached, the amylase will stop working. Hypothesis: The reason I believe that as the temperature of the starch and amylase increases, as will the rate of the reaction, is because of the collision theory. ...read more.

Middle

Ensure that the temperatures of the water baths and ice bath are kept as constant as possible. Ensure that the starch and amylase solution is kept in the water bath in between each 30-second gap, so as to ensure a constant temperature throughout. Method: Two test-tubes, one filled with 5mg of starch suspension, and one filled with 5mg of amylase solution, are both put into a tub of ice. Whilst these are left inside the tub to cool down, droplets of iodine solution are put in each cavity of the spotting tray. Once the temperatures of the starch and amylase solution have become constant, then they are mixed together, and a droplet of this solution is then added to the first cavity of iodine. The test-tube is then put back in the ice tub, and then every 30 seconds, another droplet of the solution is added to a different cavity of iodine, until the purple colour that should appear, has disappeared. The time taken for the purple colour to disappear, is then recorded. ...read more.

Conclusion

This of course was incorrect, because once it reached 30?C, the amylase did not stop working, it just became less efficient. One thing I noticed whilst looking at the results table, was that as the temperature increased by 10?C, the rate of reaction doubled. This would explain why the graph increases steadily at the beginning, and then starts to steeply increase. Errors and Improvements: Some of the results may have been inaccurate, due to the 30-second range in between each time another droplet is added to a cavity of iodine. This could have been improved upon, by making it only a 5-second range for example, because the exact time when the starch disappears would be closer to the time recorded than with a 30-second range. An example as to why a couple of the results may not have been proportional, is when the starch and amylase were put in the ice tub. It is very unlikely that the solutions would have reached 0?C and remained constant. This could have been improved upon, by putting the solutions in a freezer instead, because it would keep to the same temperature throughout. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Investigating Enzymes

    Although enzyme catalysis greatly reduces this, some energy is still required. Because of this the reaction is still unable to happen below a given temperature (this varies depending on the type of enzyme and reaction, as does the maximum temperature).

  2. Activity of Diastase On Starch

    The reaction mixture was then titrated with quantitative benedict's. Following titration readings were obtained. Volume of reaction mixture used at 20�c Titration no. initial reading Final reading Volume of reaction mixture used 1 1.00 7.5 6.5 ml 2 7.5 13.9 6.4 ml 3 13.9 20.3 6.4 ml Mean volume of

  1. Factors Affecting Enzyme Activity

    Further Theory The mechanism by which the enzyme breaks down its substrate relies heavily on collision theory. This theory states that for the substrate to be broken down by the enzyme the two must collide. However, this is more complicated than it sounds.

  2. What factors affect the activity of urease? What are the kinetics of the urea-urease ...

    Moreover, when they do collide, they do so with more energy. This makes it easier for bonds to be broken so that the reaction can occur. As temperature continues to increase, the speed of movement of the substrate and the enzyme molecules also continues to increase (increase in kinetic energy).

  1. Investigating the effect of enzyme concentration on the hydrolysis of starch with amylase.

    Reactions can only occur when particles first collide. The collision present must have enough energy impact to overcome the Activation Energy; they must also have a proper positioning to be effective. Increase the concentration of the enzyme and there will be more molecules of that enzyme in the solution.

  2. Investigating Enzymes.

    Stop Watch Suction Tube Enzyme Pipette Cuvette Colorimeter Water Hydrogen Peroxide Guiacol Method Used: 1. Set up a Vernier Lab Pro with TI 83 calculator. 2. Set Up an colorimeter 3.

  1. Factors affecting Osmosis in Potato Tissue.

    is relatively fast, it is not so fast that it is impossible to measure the rate of reaction. These are the results for 1 molar hydrochloric acid and 8cm magnesium ribbon. Time (s) Volume of hydrogen (cm ) 10 8 20 14 30 22 40 28 50 35 60 44

  2. Catalase activity in the liver

    in my experiment is by performing an all or nothing experiment. The way I will do this is by, firstly heat 2ml of stock solution in a test tube to 70?C; I will do this because I have learned that at this temperature the enzymes denature or die.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work