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Factors affecting the rate of enzyme mediated reaction.

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Introduction

PLAN Friday 19th April 2002 Catalase Coursework. Title: Factors affecting the rate of enzyme mediated reaction. Aim: To find out how the concentration affects the rate of reaction. Introduction: Enzymes are proteins that control vital biological processes. They often act as biological catalysts. In the human body enzymes control the breakdown of food and reactions which make chemicals such as fats, carbohydrates, proteins and DNA. Enzymes are used today in a wide range of industrial processes. These include fermentation, baking, cheese making, tenderising meat and treating leather. The table summarises some of the enzymes used in industrial processes. The enzyme catalase is present in blood to prevent the build-up of dangerous peroxides. If a small piece of liver is added to hydrogen peroxide, a rapid evolution of oxygen is seen. The catalase acts as a catalyst and speeds up the decomposition of hydrogen peroxide. 2H202(aq) ==> 2H20 + 02(g) Catalase is an extremely effective catalyst. ...read more.

Middle

Measure out 20 ml of H2O2 using a measuring cylinder. Pour it into a test tube (make sure test tube is in the test tube rack). Using the borer, bore through the potato. Place the potato on the tile and measure out 5cm of potato. Cut the potato into 5 1cm pieces and make sure there is no skin left on the potato as it will slow down the rate of the reaction. Half full the bowl with tap water. Put the potato in with the H2O2 and quickly set up the apparatus as shown in the diagram. After 5 minutes check how much O2 has been released, this will show how the concentration affects the rate of the reaction as the more O2 in 5 minutes shows that the reaction is working faster. Empty test tube and rinse well. Measure out 16 ml of H2O2 and 4 ml of H2O using a measuring cylinder. Pour it into the test tube (make sure test tube is in the test tube rack). ...read more.

Conclusion

Using the borer, bore through the potato. Place the potato on the tile and measure out 5cm of potato. Cut the potato into 5 1cm pieces. Put the potato in with the H2O2 and H2O and quickly set up the apparatus as shown in the diagram. After 5 minutes check how much O2 has been released. Changes made after trial run: We doubled the concentration of H2O2 and chopped the potato up into 5 1 cm pieces. We doubled the concentration of H2O2 as with 10 ml you can't see the reaction as much, it is easier and clearer to get our results from 20 ml of H2O2. Prediction: I predict that the higher the concentration of H2O2 the rate of the reaction will occur faster, and the more distilled water you add the slower the reaction will occur. I think this because the catalase acts as a catalyst and speeds up the decomposition of hydrogen peroxide 2H202(aq) ==> 2H20 + 02(g) Hydrogen peroxide acts as a biological catalyst, it breaks down the potato/ living cell causing a rapid evolution of Oxygen. ...read more.

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