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Factors that Affect Enzyme Reactivity.

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Introduction

Factors that Affect Enzyme Reactivity. Hydrogen peroxide (H2O2) is a highly active chemical, often used for bleaching. It is sold as a 3% solution in water. Within cells, hydrogen peroxide is formed continually as a by-product of biochemical processes. Because H2O2 is toxic (poisonous) to cells, it would soon kill them if not immediately removed or broken down. In this investigation you will observe the activity of a substance that breaks down hydrogen peroxide. The substance is an enzyme, catalase, an organic compound. Materials: 3 pieces 6mm diameter liver 10 test tubes Fresh potato 10ml graduate 100 ml 3% H2O2 solution beaker scalpel Fine sand (Only to help in grinding. Sand is not otherwise involved in the experiment.) ...read more.

Middle

7. Design the data table based on the variables being studied. 8. State the purpose of this experiment. 9. Make a hypothesis for each variable studied. 10. Does your body contain catalase? Explain your reasoning. 11. Write the balanced chemical reaction. Procedure: 1. Arrange the test tubes in the rack and number them for identification. 2. Pour 2 ml hydrogen peroxide (H2O2) solution into each of the tubes. After each of the following steps, record your observations. Compare observations on different tubes frequently. 3. Using forceps, select a small piece of fresh liver and drop it into a test tube. Be sure to record the test tube number, contents, and the results in your data table. ...read more.

Conclusion

with sand. Place the potato-sand mixture in a test tube. Discussion: 1. What caused the reaction when you put the liver into the test tubes? 2. Explain why there was a difference in the amount of bubbles from the whole piece of liver and the ground liver 3. How could you prove that it was not the sand that caused the reaction but the liver or potato? 4. How do you explain the difference in activity resulting from the boiled liver? 5. What evidence do you have either for or against this reason? 6. What do you think would have happened if you had boiled some potato and put it into hydrogen peroxide? 7. Which of the materials used seems to contain more catalase, potato or liver? Why do you think this? REMEMBER TO FOLLOW THE FORMAL LAB WRITE-UP GUIDELINES WHEN YOU WRITE YOUR REPORT. ...read more.

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