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Find out the effect of changing solute concentration on osmosis in potato tissue.

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Introduction

Osmosis Aim The aim of the investigation is to find out the effect of changing solute concentration on osmosis in potato tissue. Prediction I predict that if the solute solution is more concentrated than the potato, the potato chip with the more water potential will lose water, so if the potato chip is placed into a dilute solute solution, the potato will gain water. Here's the example- if the original cells is 5g then and placed into a diluted solute solution the mass will raise to 7g. And if the potato chip is to be placed into a concentrated solution, the potato chip's mass will drop to around 3g. I predict this because of the process of osmosis. Diffusion is the movement of particles from a region of its high concentration to a region of its concentration. Osmosis is a special kind of diffusion which involves water. The definition of osmosis is - 'the net movement of water molecules from a region of high water potential to a region of low water potential across a semi- permeable membrane.' This is a diagram showing where water from the dilute solute solution on the left passes through the semi- permeable membrane and moving into the high concentrated solute solution on the right. ...read more.

Middle

We will use a 50ml Burette to measure the amount of water and sucrose we will add in our mixture of solutions. We will not use a measuring cylinder because it doesn't have a 0.1ml + or - measurement unlike the burettes. I chose to measure 9 different concentrations because its not too little and 9 makes perfect for all the mixtures shown below: Concentration (mole) 2.00 1.75 1.50 1.25 1.00 0.75 0.50 0.25 0.00 Water (ml) 0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 Sucrose (ml) 2.00 1.75 1.50 1.25 1.00 0.75 0.50 0.25 0.00 To heat the test tubes and keep them constant at the optimal 37c, we will need to use a water bath We will repeat the investigation 3 times for reliable results. And plot down all results on a table like the one shown on the next page. After doing all three we will use the average amongst the three. The average will lead us into finding the pattern of this investigation. Concentration of sucrose Solution (m) Mass of Potato Tissue (g) Average Mass of potato tissue (g) Difference in mass (g) Trial 1 Trial 2 Trial 3 Before After Before After Before After Before After 2.0 1.75 1.5 1.25 1.0 0.75 0.5 0.25 0.0 Procedure 1. Set up apparatus like shown below 2. Fill one beaker with sucrose (2mole) solution 3. ...read more.

Conclusion

Evaluation I think my method was good because it supported my prediction. There was some room for improvements though. Because we found that some are anonymous results. We tried to figure why this might have happened. I think using the burette was one of our most accurate and reliable piece of equipment. It can measure up to 0.1ml. There was nothing wrong with that. Water bath can be put into 37c with no problems. It's reliable equipment. There's even a thermometer next to it so u can be extra accurate. A borer was easy to use and reliable because of its cylinder shapes, can produce same shaped potato cuts each time keeping the surface area the same. I used 9 different concentrations because it can used to make 9, easy to make, concentrations. Plus 9 concentrations are enough to make a reliable fair test. We repeated the test three times because of reliability. One test is likely to make a mistake. The reason why I think I got the anomalies is because, while putting them in and taking them out we had problems. Not enough people to plot them in at the same time however, I found out a way to solve this. This is how I thought it should be done. When plotting them in if we together had four hands we could have delayed the other lot about ten seconds then we get them out ten seconds earlier. ...read more.

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