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Find out the relationship between surface area and the rate of oxygen produced when potato is placed in hydrogen peroxide.

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Introduction

Biology Investigation Investigation I am trying to find out the relationship between surface area and the rate of oxygen produced when potato is placed in hydrogen peroxide. Introduction I will find out that if the surface area of a potato cylinder changes, will the amount of oxygen produced by the enzyme catalase be affected. Prediction I predict that as the surface area of the potato cylinder increases, the quicker the reaction between the hydrogen peroxide and potato cylinders and therefore more oxygen will be produced. One reason for more oxygen being produced as the surface area increases is that more active sites are present which increases the chances of catalase breaking down the hydrogen peroxide into oxygen and water. Scientific Evidence to support my prediction The speed of a reaction can be affected by a few factors. One of these factors is catalase. Catalase is one type of enzyme. An enzyme is also a type of catalyst which can increase the speed and rate of a reaction. Enzymes work because they are covered in active sites which allows them only to lock into certain molecules. This process is the lock and key theory. The lock and theory works when something has an active site and only a certain substrate will fit into this active site. ...read more.

Middle

The tap burette will be filled with water to see how much oxygen displaces it in 3 minutes 4) I will then use a size 6 cork borer to extract the potato cylinder from the potato 5) The potato cylinder will then be placed into a test tube of 20ml of hydrogen peroxide 6) The experiment will be allowed to run for 3 minutes 7) The amount of water remaining in the tap burette will be taken away from the original amount giving me the amount of oxygen produced 8) This will be repeated twice and the results will be recorded and the average will be worked out 9) The experiment will then be carried out with different lengths of the same size potato cylinder which increases the surface area. Diagram Intepretation of results Through reading the line of best fit on my graph, I have realised that as the surface area of the cylinder increases, the volume of oxygen produced increases. This has happened because when there is a large surface area of the potato cylinder, there are more active sites exposed increasing the chance of the active site fitting with the substrate causing the enzyme to break down the hydrogen peroxide. My graph shows that as the surface area increases, the volume of oxygen produced increases. ...read more.

Conclusion

Another experiment to further my results is the effect of temperature on enzymes. The experiment with temperature would involve me doing an enzyme-controlled reaction at different temperatures to see how temperature affects the rate of a reaction. For example I would see how fast the reaction is going at 10C, 20C, 30C, 40C, 50C and this would show if there is any correlation between increasing the temperature and the speed of a reaction. This could help the experiment that I did because I would have known that temperature can affect the rate of a reaction and therefore I would have controlled the temperature. The other experiment with pH would have involved me doing an experiment with enzyme solution in different test tubes. The test tubes would have contained an alkali, an acid, water and boiled water. Photographic film containing gelatin will be placed in each of the test tubes. If the gelatin has been digested the film will turn colourless. If the gelatin has been digested the protease would have worked. This experiment will show the effects of pH conditions of an experiment and how pH conditions can affect certain experiments. This experiment would have helped the experiment I did because I used an acid in my experiment and this could affect what I was trying to find out. ...read more.

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