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How catalase affects the speed of breakdown of hydrogen peroxide.

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Introduction

Catalase investigation Aim: How catalase affects the speed of breakdown of hydrogen peroxide. Introduction: Enzymes are biological catalysts in a way like protein molecules and also they make the rate of reaction increase that occurs in a living organism. "A catalyst is a substance that increases the rate of reaction" while also it doesn't get used up in this process and it is found in our liver. In this experiment the catalyse which is hydrogen peroxide can be used again and again in order for the increase of "rate of conversions of the reactants into products". For this experiment the equation to describe this reaction is: Hydrogen Peroxide Water + Oxygen Next I looked at how the following factors within the experiments affect the rate of reaction/ volume of oxygen evolved in a Catalyse Reaction: Concentration: In order for the reaction to become more faster the concentration in the solution (in this occasion hydrogen peroxide solution) must be increased causing the particles to get more crowded which will cause them to collide into each other more. This means more number of successful collisions making the rate of reaction faster. Because more collision is taking place and there is a high amount of concentration the volume of oxygen will be evolving faster. ...read more.

Middle

As soon as that is done I will start the time on the stopwatch and I will start of by taking the measurement from 0 min. I will take the reading every four minutes. List of apparatus: * Safety goggles * Potato * Test tube * Delivery tube * Measuring cylinder (large and small) * Flask * Hydrogen peroxide * Tile for cutting potato * Thermometer * Knife * Ruler * Stop watch * Pen/pencil. Safety: I need to wear safety goggles but before starting. I also did some research on the chemical hydrogen peroxide to see what I'm dealing with. The chemical is corrosive and irritant meaning it can cause skin burns and irritate yours eyes. If the following types of problem occurs than they have to take the following emergency actions: In Eye: Gently put water in to your eye and then go hospital immediately. Swallowed: Wash out your mouth, drink a glass of water and do not try to vomit and then go hospital immediately. Spilt on skin: Wash that area with water and if you still have burns, blisters or rash than go hospital immediately. Fair testing: Before starting the experiments I will clean all the apparatus after each experiment, measure the oxygen from the meniscus point, I will cut each potato into exactly 1cm , for each experiment I will add 30ml of hydrogen peroxide solution and all the experiments will last 24 minutes so that I have done fair tests. ...read more.

Conclusion

Evaluation: I am generally satisfied with my results as they prove right my prediction. The points on my graph are close to the line of best fit which shows that the results are reliable. The procedure that I carried out was suitable for the experiment and it also shows by looking at my results that it was suitable. I must admit that my results are not 100 percent accurate because I did find it hard to cut the potatoes into exactly 1 cm cubed and also another thing is I found reading the amount of oxygen produced from the measuring cylinder from eye level quite difficult but I did get used to reading it as I did a lot of eye level reading. To make the results more accurate and reliable could have been achieved by making a machine cut the potatoes exactly into 1cm cubes which can not be achieved by first time human cutters. Also the temperature was not kept constant because I was working with 30 or more students in the same room by which they all emit a little heat from their bodies which add together to give lots of heat in to the potatoes. So it would help if we worked in separate rooms to keep temperature constant. Akul Cara 10y1 ...read more.

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