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How does temperature affect the rate of reaction between catalyse and Hydrogen Peroxide.

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How does temperature affect the rate of reaction between catalyse and Hydrogen Peroxide. Aim: To find out the factors affecting the rate of reaction of catalyse and hydrogen Peroxide. Key Factor(s) which will affect the experiment- In order for me to methodically and fairly find out my aim in this experiment, I will need to change only one factor or variable throughout my investigation. The reason for this is so I no what factor will be causing the reaction on the experiments. So the key factor (or the only factor I will intentionally change) in this investigation will be temperature. This is so I can find out how temperature will affect the rate if reaction between catalyse and hydrogen peroxide. I will change and use temperature in my experiment by using water baths. I will place some liver in a beaker and put it in a water bath. I will use 5 water baths each set to different temperatures. This will then heat up the liver. Other factor(s) which will affect my experiment- I have to be aware of other factors which may affect my experiment(s) as I will want to control these so all conditions are the same for each experiment. So in addition, I will not change these factors only control them, so I can see clearly how temperature alone is affecting the experiment(s)........ The amount of liver: this will affect my experiment in the way that, if I have more liver there will be more particles to collide with one another. ...read more.


Also the other reason for why the rate of reaction will increase is because some particles collide but do not have enough energy to cause a reaction. Therefore if the particles have more energy then they will collide harder as they go faster and there bang will cause a reaction. From this information that I know I could predict that as I raise the temperature of the particles (as I raise the temperature of the liver) there will be a greater reaction as the particles will have more energy to collide. But, I also know that all enzymes are proteins. At high temperatures the protein breaks down. Now the active site has changed, now the substrate will not fit. The enzymes have now been denatured, and in affect will no longer work. (See diagram below). I now will have to change my prediction from: The more I heat up the particles in the liver; there will be a greater reaction. This is because as I heat up the particles they will have more energy and therefore travel faster and collide more. My prediction will now be: I think as I increase the temperature of the particles in the liver, there will be a greater reaction. But once I exceed a certain temperature, then the protein will break down. This means that the substrate will no longer fit. And therefore the rate of reaction will lower. ...read more.


So from my results I think I can draw a firm conclusion. Conclusion- I think that an important and key factor that very much affects the rate of reaction between Catalyse and Hydrogen Peroxide is temperature. The rate of reaction increases up until 50�. After this temperature (so above 50�), the enzymes in the liver get denatured, and therefore the higher the temperature past 60�, the lower the rate of reaction will be. So temperature in this investigation affected catalyse (catalyse in the liver) and hydrogen peroxide by affecting the rate of reaction between the both, by either slowing down the rate or making the reaction faster. I could extend this investigation, which would still aim to find out more about the influence of the same factor, by looking at higher temperatures and/or look at different reactants. By extending my investigation I will again a greater understanding of my investigation and aim. I could also extend my investigation by looking at time. I could see how long (at different temperatures) it takes for the two substances to stop reacting. I could also extended my investigations by looking at different substances that contain catalyse. Or I could look at the surface areas and/or weight of the substances (e.g. I could use a chunk of potato and weight it or look at its surface area). If I was to extend this investigation, in any way, I would make sure I used accurate measurements e.g. 0.5ml of liver rather than 3 drops. And still continue to make my experiments as fair as possible. ...read more.

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