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How Does The Concentration of a Sucrose Solution Affect The Mass of a Potato Chip?

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Introduction

Biology Investigation Into Osmosis: How Does The Concentration of a Sucrose Solution Affect The Mass of a Potato Chip? Introduction I am going to investigate how changing the concentration of sucrose in a solution will alter the mass of a potato chip placed in it for a certain time. To do this I need to set up a set of experiments where potato chips of known masses are placed in sucrose solutions of different concentrations and left for a time, after which their mass is recorded again. By carrying about the method below I will be able to observe and record the changes in a potato chip's weight that occur with different concentrations of sucrose solution then calculate the average percentage change in mass of each set of chips and draw a line graph. Method (For Graphical Illustration, see next page) 1) I will cut out twelve potato chips, with a scalpel, all with the identical dimensions of 8x7x20mm. 2) I will weigh each of the potato chips and record their masses in a table, labelling half of them A and the other half B, a pink streak of felt tip pen will denote B. 3) I will attach all the A chips to their corresponding B chips with a pin. 4) At one minute intervals I will place each of the pairs into six different boiling tubes containing sucrose solutions, varying from a concentration of 0.1M to 0.6M and increasing in 0.1M intervals. 5) After twenty minutes is up for the first pair I will carefully remove them with a mounted needle and dry each chip with three strokes of filter paper to remove excess solution. 6) I will separate the A chip the B chip, weigh them and record their new masses in a table. 7) I will reattach A to B and replace it in the sucrose solution. 8) I will repeat numbers 5-7 for each of the six chip pairs. ...read more.

Middle

Percentage change in mass Average percentage change in mass A B A B A B A B 0.1 1.40 1.42 1.74 1.68 0.34 0.26 24.3 18.3 21.3 0.2 1.41 1.40 1.52 1.64 0.11 0.24 7.8 17.1 12.5 0.3 1.50 1.44 1.58 1.72 0.08 0.28 5.3 19.4 12.4 0.4 1.40 1.40 1.33 1.49 -0.07 0.09 -5.0 6.4 0.7 0.5 1.33 1.41 1.41 1.34 0.08 -0.07 6.0 -5.0 0.5 0.6 1.39 1.40 1.30 1.22 -0.09 -0.18 -6.5 -12.9 -9.7 NB. To work out the percentage change in mass, I took the change in mass, divided it by the initial mass and multiplied the answer by one hundred. Graph Notes I have decided to put all the data on one graph so that I can compare the two sets more effectively. Line One is the line of best fit for the results for the chips' percentage mass change after twenty minutes. Line Two is the line of best fit the results for the chips' percentage mass change after two days, a time span of approximately 52 hours. Point P denotes the water potential of the cells while results that are circled are ones that I believe do not entirely correspond with the overall pattern. Red crosses indicate change in mass after twenty minutes, blue points show change in mass after two days. Conclusions Part One-Potato Chips After Twenty Minutes: The graph of average percentage change in mass against sucrose solution strength shows a rough pattern of negative correlation, the change in mass is high and positive when the solution is weak, the highest value being 10.1%, and gradually decreases in value until the last result where the change in mass, measured as -4%, is negative. I was able to draw an approximate line of best fit onto the graph and it crosses the x-axis when the sucrose solutions concentration is around 0.5 Molar. Therefore, I have found out that before this point, as the solution gets stronger the chip gains progressively less mass and after this the point the chip starts to lose mass. ...read more.

Conclusion

To extend my investigation further I could try repeating the experiments using a different solution such as glucose or protein or I could use a different source of plant cells and see if their water potential differs from the potato. It might be interesting to investigate the relationship between the mass change and the surface area of a potato chip while keeping the solution's concentration constant. I might also be able to investigate the effects of changing the temperature of the solution. If I wanted to find out how the change in mass changed over time I think I would make up many identical solutions and cut out chips that were identical in every way. I could then measure one batch after a certain length of time then dispose them while still leaving many other unopened sets that I would measure later. The ideal investigation would be to do the experiments on a single plant cell, however I no not believe this is possible in practice unless you have some very hi-tech and expensive equipment. I do not think that my conclusion could always be counted on, with regards to the numerical data values, because although I thought my results were quite accurate and they agreed with those collected by others, I would need to collect more evidence and do more experiment and repeats to verify it. There is also the possibility that the measuring balance malfunctioned and gave incorrect results, either at the start or the end of the experiments, and I did not make up the solutions myself so some of them could have been prepared incorrectly and so given false results. Finally, in the period when I left my experiment at school it could have been tampered with my someone. However I believe my overall conclusion and explanation of the pattern is the right one as Biology For Life describes a similar, more basic experiment. It relates it to plant cells and gives the same reasons for the occurrences as I have said in my prediction and conclusion. ...read more.

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