• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

How quickly does amylase break down starch when we change the temperature.

Extracts from this document...

Introduction

GCSE coursework science-planning strand Jack Darby 10F1 Miss Riaz Title of activity How quickly does amylase break down starch when we change the temperature. Instructions List I gathered the equipment shown below. I put 3 drops of iodine in each chamber of the spotting tile. I boiled the kettle and mixed together water from the kettle with water from the tap so the water reaches the correct temperature. The water was put into a beaker. 5ml of starch was put into a test tube. The test tube was placed into the beaker. When the water in the beaker was at the required temperature the stop clock was started. After one minute 1ml of amylase was put into the test tube with the starch using a syringe or pipette. As soon as the amylase and the starch had mixed a sample was taken from the test tube using a pipette and mixed with the droplets of iodine in one of the chambers of the spotting tile. ...read more.

Middle

I think that as the solution gets higher than that temperature the reaction will either be very quick or very slow depending on whether the reactants become denatured or not. I predict that the line on the graph will go up and then dip if and when the reactant denatures. Range of results I intend to take I intend to take results for 20,25,30,35,40,45,50,55,60 degrees Celsius. I will repeat my experiment. I think that there will be a wide range of results because as the temperature of the amylase changes the rate of reaction between the amylase and starch will change. I think there will be anomalous results because I think that at some point the reactants will denature and the reaction will be very slow. Scientific knowledge to explain your plan and prediction The amylase works best at around 37 degrees Celsius because that is body temperature. ...read more.

Conclusion

If the amylase has completely broken down the starch the iodine does not change colour when the amylase and starch solution is added to it. Diagrams to illustrate detailed science Explain how you will make sure your results are precise and reliable I will make sure my results are precise and reliable by recording my results accurately. I will make sure that the spotting tile and the test tubes are cleaned thoroughly after each experiment. I shall measure the temperature accurately and use the same equipment throughout the course of the experiments. I will make sure I am not biased when judging when the starch has been broken down. I shall repeat the experiment to ensure the results give a more precise indication of the reaction rate between amylase and starch. I will use a syringe instead of a measuring cylinder because this means that the measurements are much more reliable and accurate. I will use a thermometer which can measure temperature precisely. ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Rates of Reaction - The Iodine Clock

    Wash out all apparatus used to hold reactants with distilled water so as to prevent contamination. This should also be done between repeats. Prepare fresh 1% starch solution. When performing a set of experiments (i.e. the entire investigation of one variable)

  2. The Iodine Clock Investigation

    Apparatus: * H2O2 - 2 Vol. * KI -0.1M * H2SO4 - 1.0M * Na2S2O3 -0.005M * 2% starch solution * 50ml burettes (x5) * conical flasks * volumetric flasks * stands and clamps * funnels * stopclock * water bath (i.e.

  1. Activity of Diastase On Starch

    Iodine works by binding to the starch. When iodine binds to the starch it will more than likely interfere with diastase activity (this effect is called steric hindrance, literally the iodine would block the starch from binding at a molecular level)

  2. How quickly does amylase break down starch when we change the temperature.

    Enzymes are biological catalysts and all catalysts need an optimum temperature in order to break down the reactant as quickly and as effectively as possible. Enzymes are protein molecules.

  1. How fast is starch broken up by the catalyst amylase.

    - To see if we had got the temperature a thermometer was used.

  2. The effect of temperature on the reaction between amylase and starch

    This was shown in my graphs as well. At 70�C the average reaction time increased to 134 seconds and the rate of reaction decreased to 0.007 seconds. These results show the beginning of the denaturing of the enzymes. Raising the temperature had two effects.

  1. The Iodine Clock

    this will make the experiment faster and so will make more time for repeating the experiment. This also means that I will be investigating how the sodium thiosulphate affects the time for the solution to change colour, as in my preliminary work it was shown that as the concentration of

  2. Catalase activity in the liver

    The temperature of the stock solution will have to be taken to see if it has reached my wanted temperature to do this I will use a thermometer (measured in ?C).

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work