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How Temperature Affects Catalase Activity.

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How Temperature Affects Catalase Activity. Aim: In this experiment I aim to investigate how altering the temperature exposed to the yeast catalase and the hydrogen peroxide, will affect the reaction and the gas product produced. Scientific background knowledge lets us know that there are 6 variables that usually affect the rate of a reaction, and they are as follows: * Concentration of enzymes * Concentration of hydrogen peroxide * Temperature condition of reaction * Pressure condition of reaction * Physical state of solid substrate (e.g. surface area of particles) [Some material reproduced from "Biology 1" endorsed by OCR] In order to ensure fairness and accuracy, I will see to it that all the necessary and vital measurements are taken precise to the mark, and I'll aim to maintain all other latent variables constant. Hypothesis: Via my observation, I intend to prove that as the temperature of both the yeast catalase and the hydrogen peroxide id equally increased, the rate of which the reaction will take place will also be increased. When the enzymes reach the optimum temperature level, the slightest escalate in temperature will leave the enzymes permanently denatured. So to summarise my predictions, my expectations fro this investigation are as follows: * Low temperature will equal slow reaction * Any increase in temperature will mean an increase in reaction * Temperature exceeding the optimum level will result in the denaturing of the enzymes. Introduction: An enzyme's speed of action expressed as its turnover number. ...read more.


3. Analysing of evidence (and drawing conclusions) Graph 1 This graph illustrates the relationship and trend gained when the temperature of enzymes catalase is increased. This graph basically shows us that from a low temperature, the time taken to get to 50ml will simultaneously be increased via the raise of heat applied, this will continue until the temperature becomes too extreme for enzymes to work sufficiently. Therefore the time will eventually decrease in speed, and takes longer to get to 50ml until it finally stops. Although the graph supplies us with all the information stated above, it does not specify the realationship between the temperature and the rate of reaction. In order to find the rate of reaction (the speed of which the product is produced) there is a special formula to follow. Rate: volume of gas produced /time or in this case 50/time This table below displays the results of the same experiment, but with and extra column representing the rate. Temperature (oc) Volume of hydrogen peroxide Volume of yeast catalase No. Of trials Time taken for gas to expand to 50 ml Rate of reaction 50/ time taken In minutes In seconds 5 15ml 15ml 1 3mins 5 sec 205 Seconds 0.24 10 15ml 15ml 1 2mins 30 sec 150 Seconds 0.33 20 15ml 15ml 1 1mins 45 sec 105 Seconds 0.48 30 15ml 15ml 1 1mins 30 sec 90 Seconds 0.56 40 15ml 15ml 1 0mins 44 sec 44 Seconds 1.14 50 15ml 15ml 1 1mins 37 sec 97 Seconds 0.52 60 15ml 15ml 1 ...read more.


However, after reviewing that idea it occurred to me that there would still be a delay regardless. The only improvement would be the shorter time length it would take to add the bung on the flask. -It became clear that the only way I could rid of this problem would be to get an apparatus where the bung doesn't have to be removed from the volumetric flask, yet the solutions can still be combined and mixed and the gas given off can still be measured by the gas syringe. For instance, if the bung had two holes intended in it, were one hole was connected to a tap, which would release the hydrogen into the catalase, and the other connected to the gas syringe, which would collect the gas discharged. This process would surely affect the accuracy and reliability of the results. Reliability My conclusion is: Rate oo temperature Being that the shape of my second graph is just as I had predicted and explained, I can confidently say that the results of the experiment supports my hypothesis very well and is really quite reliable. Further investigation: As we know there are several things that affect the rate of reaction and temperature is just one of them. If I were to further investigate how the rate of a yeast catalase reaction could be affected, I would probably investigate concentration. It would be interesting to see how increasing the amount of particle available can affect the speed of a reaction. ...read more.

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