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How Temperature Affects The Structure Of The Cell Membrane.

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Introduction

How Temperature Affects The Structure Of The Cell Membrane. In This Experiment I Will Use Beetroot To Examine How Temperature Affects The Structure Of The Cell Membrane. The membrane is made of phospholipids and proteins. Proteins have a specific shape and so will be affected by temperature when it goes high, just like enzymes, which are also proteins. Therefore when the temperature gets too high the protein will denature (change shape). All that happens when phospholipids are in high temperature is that they start to vibrate which does not affect the membrane being a consistent barrier. ...read more.

Middle

Firstly I will use cork borer number 1 to get a cylindrical shape of beetroot, which I will cut to 3cm in length using a scalpel. By using a cork borer I will be ensuring that the surface area is the same each time, meaning there will be the same amount of membrane each time. I'll then make up a 200cm� solution of distilled water (200cm�) and two-pH7 buffer tablets in a beaker, which will keep the pH constant, which will make it a fair test. I will use a measuring cylinder to make sure that the measuring of the distilled water is accurate. ...read more.

Conclusion

After this time is over I'll remove the test tube from the water bath and pour the liquid minus the beetroot into a cuvette, which I will then put into a colorimeter and take down the per cent transmission of light. I will repeat the experiment 3 times and take the average result, to make it more accurate. I will do a control experiment for each of the temperatures using 10cm� of clear distilled water with pH buffer in it, which should give 100% transmission of light. These will contain no beetroot and will be done at each temperature, put into a cuvette and then put into the colorimeter. ...read more.

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