• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

How will exposure of E. coli to different kinds of light affect its growth? Hypothesis: Under the exposure of no light, E. coli will thrive and grow at maximum speed

Extracts from this document...

Introduction

Abdel Rahman Negm I.B. Biology H1 Research Project #1 Escherichia Coli and Light November 5, 2004 Abstract: There are thousands of kinds of bacteria in the world, each one with its own special unique characteristics. In this experiment, one will work to discover certain aspects of the bacteria known as Escherichia Coli (E. coli). E. coli is not necessarily deadly bacteria, but it can cause bloody diarrhoea and maybe even kidney failure. This awful kind of bacteria can be transmitted to humans through uncooked meat or drinking raw milk or contaminated water. One will use different combinations of E. coli (growing on agar) and light to explore and understand what kind of habitat is more suitable for it and which habitat E. coli favors for growth. Through this experiment, one will learn and understand more about this harmful kind of bacteria and learn about the conditions (of light) that it favors the most. Problem: How will exposure of E. coli to different kinds of light affect its growth? Hypothesis: Under the exposure of no light, E. coli will thrive and grow at maximum speed. It's very well known that most bacteria does thrive and flourish in dark, moist conditions (such as one's arm pit; after a long, sweaty jog outside in the heat, one's body will most probably give off a bad smell known as body odor and this is because of the bacteria living in the dark, and now wet, conditions of the arm pit- the bacteria grows faster and gives off that odor.) ...read more.

Middle

Note: if while the water is boiling, it starts to flow out of the beaker, then decrease the temperature to accommodate for this and to stop this overflowing from happening. 7. Then quickly take the solution and pour and distribute it equally between each of the four Petri dishes. 8. Quickly cover up the Petri dishes and leave them aside to allow the agar to cool and harden. 9. Once this is accomplished, take your intraocular loop and sterilize it by placing it directly in the flame of a match to eliminate any foreign and unwanted substances that may be found on the loop. Note: Leave the loop in the flame until it turns orange. 10. Now, take the gloves and put them on and take the bacteria source, open it carefully, and move the loop across the surface of the bacteria such that it becomes contaminated. 11. Once this is done, close, the bacteria source, then take the loop and move it across the surface of the agar directly on the center point of each of the Petri dishes. 12. Close the Petri dishes and sterilize the loop again to now kill and destroy the harmful bacteria. 13. Now completely seal each of the Petri dishes to prevent the bacteria from escaping by taping the lid and bottom of the dish containing the agar shut- tape it all around its edges and leave the tops and bottom of the dish clear for viewing. ...read more.

Conclusion

Also, when the agar was left uncovered for that period of time, a foreign substance also might have gotten in causing this. Also, after day 2, the data table reads N/A in every column for, in the Petri dishes of Light 1 and Light 2, a fungus began growing and some of the agar began turning black, ending my experiment and my ability to record any more data. The reason for this fungus is most probably the same as the Dark 1 Petri dish- a foreign substance/organism made it in the Petri dish and on to the agar. Conclusion: According to the little amount of data acquired, one could say that the dark Petri dish did grow better than the light Petri dish, but then again, it is not accurate and right to say that for the size of the errors were too large and so the limitations and weakness of the errors were large. And so my confidence and certainty towards my results isn't very high. These limitations had to do with flaws and errors in the procedure; I filled the Petri dishes with the agar right after I got the Petri dish without sterilization of any sort and so this dramatically affected my results- it contaminated and ruined my bacteria and agar causing the end of my experiment. For improvements for any future experiment, sterilization of all equipment, tools, and utensils will be a priority and first thing on the list for as one has seen, un sterile objects give very inaccurate results. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Living Things in their Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Living Things in their Environment essays

  1. Marked by a teacher

    Escherichia coli and antibiotic resistanceIntroduction:Escherichia coli, short E. coli is an important bacteria that ...

    5 star(s)

    A native organic population, whether be E. coli bacteria or insects is most susceptible to the new a type of foreign invasion because the organic population has previous experience with the new force and thus has no defense against it.

  2. Investigating the effect of four antibiotic agents on gram positive and gram negative bacteria.

    * Tape petri dishes after inoculation and label the base clearly with name, date and nature of inoculum to avoid microorganisms escaping into the environment if the dish should be dropped and pathogens can be easily identified if contamination occurs.

  1. Branded Bleach is more effective at killing E. coli than Non branded bleach - ...

    The log stage is the stage required for the experiment to be successful as the death of the bacteria and zones of inhibition is due to the action of the bleach and no other limiting factors. However as these limiting factors begin to take effect the graph begins to level

  2. Investigating Seed Germination. Hypothesis If there is water, oxygen and a suitable ...

    The length of each seedling will be measured using a vernier caliper, with units in cm. Controlled Variables: --> Volume of Water - The volume of water poured per day onto the seeds each day should remain the same, as if the volume changes, the volume of water imbibed by

  1. An investigation into the antibiotic effects of penicillin and streptomycin on the bacterium Escherichia ...

    Gram-negative bacteria when developed, such as ampicillin which can treat both Gram-positive bacteria and Gram-negative. Penicillin works by forming peptidoglycan cross links in the bacterial wall, which causes the cell wall to weaken when it tries to divide, causing cytolysis.

  2. The main aim in the life science lessons is to learn how to handle ...

    Next we label one of the quarters as 'before' and the other as 'after'. Thereafter we opened the dish at forty-five degrees and poured in the melted LB agar into the petri dish until the dish was 3/4 filled. We covered the dish immediately and swirled it gently until the LB agar covered the base completely.

  1. To see how Blowfly larvae (Calliphora) react to light.

    Furthermore, I predict that the speed of the larvae will be dependant on where they are situated. If they are nearer to the light source, they will move away from it faster, slowing down as the intensity of the light decreases.

  2. Introduction to bacteria

    But sometimes people become so sick from a bacterial disease that hey require medical treatment. Antibiotics and other antibacterial drugs are the major weapons against disease-causing bacteria. Antibiotics work in a number of ways to kill bacteria or suppress their activity.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work