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How will exposure of E. coli to different kinds of light affect its growth? Hypothesis: Under the exposure of no light, E. coli will thrive and grow at maximum speed

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Introduction

Abdel Rahman Negm I.B. Biology H1 Research Project #1 Escherichia Coli and Light November 5, 2004 Abstract: There are thousands of kinds of bacteria in the world, each one with its own special unique characteristics. In this experiment, one will work to discover certain aspects of the bacteria known as Escherichia Coli (E. coli). E. coli is not necessarily deadly bacteria, but it can cause bloody diarrhoea and maybe even kidney failure. This awful kind of bacteria can be transmitted to humans through uncooked meat or drinking raw milk or contaminated water. One will use different combinations of E. coli (growing on agar) and light to explore and understand what kind of habitat is more suitable for it and which habitat E. coli favors for growth. Through this experiment, one will learn and understand more about this harmful kind of bacteria and learn about the conditions (of light) that it favors the most. Problem: How will exposure of E. coli to different kinds of light affect its growth? Hypothesis: Under the exposure of no light, E. coli will thrive and grow at maximum speed. It's very well known that most bacteria does thrive and flourish in dark, moist conditions (such as one's arm pit; after a long, sweaty jog outside in the heat, one's body will most probably give off a bad smell known as body odor and this is because of the bacteria living in the dark, and now wet, conditions of the arm pit- the bacteria grows faster and gives off that odor.) ...read more.

Middle

Note: if while the water is boiling, it starts to flow out of the beaker, then decrease the temperature to accommodate for this and to stop this overflowing from happening. 7. Then quickly take the solution and pour and distribute it equally between each of the four Petri dishes. 8. Quickly cover up the Petri dishes and leave them aside to allow the agar to cool and harden. 9. Once this is accomplished, take your intraocular loop and sterilize it by placing it directly in the flame of a match to eliminate any foreign and unwanted substances that may be found on the loop. Note: Leave the loop in the flame until it turns orange. 10. Now, take the gloves and put them on and take the bacteria source, open it carefully, and move the loop across the surface of the bacteria such that it becomes contaminated. 11. Once this is done, close, the bacteria source, then take the loop and move it across the surface of the agar directly on the center point of each of the Petri dishes. 12. Close the Petri dishes and sterilize the loop again to now kill and destroy the harmful bacteria. 13. Now completely seal each of the Petri dishes to prevent the bacteria from escaping by taping the lid and bottom of the dish containing the agar shut- tape it all around its edges and leave the tops and bottom of the dish clear for viewing. ...read more.

Conclusion

Also, when the agar was left uncovered for that period of time, a foreign substance also might have gotten in causing this. Also, after day 2, the data table reads N/A in every column for, in the Petri dishes of Light 1 and Light 2, a fungus began growing and some of the agar began turning black, ending my experiment and my ability to record any more data. The reason for this fungus is most probably the same as the Dark 1 Petri dish- a foreign substance/organism made it in the Petri dish and on to the agar. Conclusion: According to the little amount of data acquired, one could say that the dark Petri dish did grow better than the light Petri dish, but then again, it is not accurate and right to say that for the size of the errors were too large and so the limitations and weakness of the errors were large. And so my confidence and certainty towards my results isn't very high. These limitations had to do with flaws and errors in the procedure; I filled the Petri dishes with the agar right after I got the Petri dish without sterilization of any sort and so this dramatically affected my results- it contaminated and ruined my bacteria and agar causing the end of my experiment. For improvements for any future experiment, sterilization of all equipment, tools, and utensils will be a priority and first thing on the list for as one has seen, un sterile objects give very inaccurate results. ...read more.

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