Lipids are made up of 3 fatty acids and glycerol. For every one glycerol there is three fatty acids (triglyceride). They are joined by the process of condensation by an ester bond.
Digestion of lipids occurs mainly in the duodenum of the small intestine. This is mainly due to the presence of bile. Bile gets added to the chyme as it leaves the stomach and passes the bile duct. The bile emulsifies the fats turning them from large insoluble molecules into small soluble molecules. Bile also helps to neutralize the very acidic chyme that leaves the stomach. The ph is lowered to an optimum ph for the lipase to operate at.
Lipases are energy stores and are sometimes called ‘nature’s storehouse of energy’. This is because, on a weight basis, they contain more than twice as much energy as carbohydrates and proteins do. Lipids also make up a major component of cell membrane, when combined with a phosphate group to form phospholipids. These phospholipids have hydrophilic heads and hydrophobic tails. Lipids are also stored under the skin for insulation and protection purposes. Lipids also surround some vital organs and provide protection for these organs, e.g. kidneys.
Hypothesis
The higher the concentration of Lipase, the greater the rate of reaction.
Prediction
I predict the higher the concentration of lipase, the greater the rate of reaction. This is because there are more active sites for the substrate to bind with the lipase so the substrate will be broken down quicker.
Things I will keep the same
- Amount of Lipase
- Amount of Bile Salts
- Amount of Fat
- Amount of Sodium Carbonate
- Amount of Indicator
Things I will change
- The concentration of Lipase
Equipment List
3 x Stop clocks
1 x Test tube stand
3 x Test tubes
1 x Pipette
1 x Glass syringe
1 x beaker of milk
1 x beaker of Bile salts
1 x beaker of Sodium carbonate
1 x Bromocresol Purple
1 x beaker of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% 100% concentrations of Lipase
Method
- Measure out 1ml of milk into the test tubes
- Add 1ml of Bile Salts into the test tubes
- Add 1ml of Sodium Carbonate into the test tubes
- Add 4 drops of Bromocresol Purple to the test tubes
- Add 2ml of 10% Lipase to the test tubes, starting the clocks when complete
- Shake each test tube 5 times every 30 seconds
- Record the time when the solution in the test tube turns yellow
- Repeat steps 1-7 changing the concentration of Lipase used e.g. 20%, 30% etc.
Results Table