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# I am going to investigate the effect of the concentration of sucrose solution on Osmosis in potato cells. I am going to do this by setting up an experiment in which I can alter dependant variables and independent variables.

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Introduction

Osmosis in potatoes To investigate the effect of concentration of sucrose solution on Osmosis in potato tissue Plan I am going to investigate the effect of the concentration of sucrose solution on Osmosis in potato cells. I am going to do this by setting up an experiment in which I can alter dependant variables and independent variables. Factors that would affect osmosis in the investigation are: * Surface area of the potato. The larger the surface area of the potato the more water it can absorb. There will be more cells to absorb more water. * Concentration of the sucrose solution. The more concentrated the solution is the less water there is in the solution. The less concentrated the solution is the more water there is in the solution. * Volume of sucrose solution. The volume of sucrose solution must remain the same throughout the experiment for the results to be fair. * Time. The longer the tissue is kept in the solution the more time osmosis has to take place. If the tissue is kept in the solution for too long the tissue will be damaged and results will be hard to obtain. But if too short, osmosis will not have enough time to take place. * Temperature. The warmer the temperature that the experiment is done in, the more energy the particles have, so they will move around in the test tube faster and osmosis will take place faster. ...read more.

Middle

Be sensible with equipment, e.g. knives. Any broken test tubes must be thrown away quickly to avoid people cutting themselves. Procedure: I collected 18 test tubes and placed each one in a test tube rack. On each test tube I placed a sticky label saying which concentration is in the test tube and which number repeat it is. I used a small measuring cylinder, which reads up to 10cm3. For the concentration of 0.0M I measured 10cm3 of distilled water. For 0.2M I measured 8cm3 of distilled water and 2cm3 of sucrose. For 0.4M I measured 6cm3 of distilled water and 4cm3 of sucrose and so on for each molarity. The readings I took from the measuring cylinder were from the bottom of the meniscus because water clings to the sides of the measuring cylinder. Before I place the pieces of potato in the test tubes with the solution I will make sure that I have filled each tube so that some of the potatoes have not been in the solution for longer than others. I will then after all test tubes have been filled with the right amount place the pieces of potato in the tubes, after these have been weighed. I will cut 18 cylindrical pieces from as few potatoes as possible with a cork borer, this makes sure they are equally round with the same circumference. ...read more.

Conclusion

* I would leave the test tubes with the potatoes in a water bath so they remain at a constant temperature. * When I dry the potatoes I should make sure that I dry them all the same amount of time. I should do this because the tissues I used could have absorbed more water from the potatoes. * To make my results more accurate I could do more concentrations than I did. Instead of 0.0, 0.2, 0.4, 0.6, etc I should use 0.0, 0.1, 0.2, 0.3, 0.4, etc up to 1M. * I could possibly use another vegetable. During my investigation nothing went wrong. There was nothing unexpected that happened. However my results were not what I expected. The results that I obtained, from the experiment to determine if my investigation was right, showed that my results from my primary investigation were inaccurate. The secondary results showed that my cell sap concentration was closer to what I predicted at the beginning of the investigation. I think that I got the results in my primary investigation because of inaccurate measuring of the liquids. From my graph I can see that there is an odd result at the molarity of 0.6M. The graph follows the pattern that I predicted in my hypothesis but the results do not follow the pattern that I predicted. The results that I obtained had a cell sap concentration at a very different molarity than I predicted. In my Biology Coursework, Osmosis In Potato Tissue Elaine Mostert 1 ...read more.

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