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I am investigating how changing the concentration of Hydrogen Peroxide affects the rate of reaction in the enzyme Catalase. This will involve placing a set quantity of liver (a source of Catalase)

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Introduction

Investigating How Concentration of Hydrogen Peroxide affects the rate of Catalase reaction. What I am investigating I am investigating how changing the concentration of Hydrogen Peroxide affects the rate of reaction in the enzyme Catalase. This will involve placing a set quantity of liver (a source of Catalase) into different concentrations of Hydrogen Peroxide and measuring how long it takes to produce an amount of oxygen. Apparatus List 6% Hydrogen Peroxide concentration, water, 1000ml glass beaker, test tube, bung for sealing test tube with rubber tube extruding from the top, stopwatch, liver, 50 cm3 measuring cylinder, measuring scales, 5ml syringe, 200ml glass beaker. Method 1. Fill the 50cm3 measuring cylinder with water and place it inverted into the 1000ml glass beaker which will also be filled with water, making sure that no gas is allowed to enter into the measuring cylinder. Fill the 200ml glass beaker with tap water. Leave to acclimatise. 2. Thread the rubber rube through the water and up into the measuring cylinder, allowing one end to be attached to the bung and the other under water. Situate the test tube in the 200ml glass beaker. 3. Place 10ml of the 6% Hydrogen Peroxide concentration into the test tube using a 5ml syringe. ...read more.

Middle

Repeats are taken to increase the accuracy of the results obtained. If any inaccurate results are taken then the two accurate results reduce the inaccuracy on the average produced. It also makes it much easier to see anomalous results. Six different concentrations of Hydrogen Peroxide are being investigated. This means the results can be plotted on a line graph with sufficient points to allow a trend to be identified. Too few points would make it hard to see the trend. Using a maximum of 6% concentration ensures that the chemical is not too dangerous to work with. Factors to keep constant Factor How kept constant Scientific reason for doing so Temperature of reaction. By placing the test tube containing the reactants into a water bath at room temperature. The hotter the particles, the more kinetic energy the particles have, which makes the probability of them colliding into each other higher. When the particles have more energy, they are more likely to react when they collide into each other. If the temperature is decreased, the particles slow down. This will make the reaction slower, as they will have less kinetic energy. Factor How kept constant Scientific reason for doing so Amount of gas collected Always get time when 25cm3 have been produced If the time is not stopped after it gets to ...read more.

Conclusion

I used 1g of liver and took the time at a volume of gas at 10cm3 Concentration Of Hydrogen Peroxide (%) Time taken to reach 10cm3 (s) 6 2.51 4 4.70 2 15.69 These results seemed plot able so I decided to stick with them and use them for the experiment. I was then left to decide what volume of gas to collect. Since I was using a 100cm3 measuring cylinder at the time of the preliminary work I tried 25cm3 because it was a large figure but small enough not to take too long. 10cm3 had worked fine, but did seem too little to be using for the experiment. I used 1g of liver and the concentrations of H2O2 stated below. Concentration Of Hydrogen Peroxide (%) Time taken to reach 25cm3 (s) 6 5.01 4 9.63 2 32.34 These results seemed to be good to plot so I chose to use them in the experiment. Prediction Since I had 3 rough readings of time for different concentrations of Hydrogen Peroxide I plotted them on a graph to see if a trend was forming so I could make a prediction. From seeing this, I predict that as the concentration of Hydrogen Peroxide decreases the amount of oxygen produced per second will decrease. Therefore the time taken to produce 25cm3 of oxygen will increase. ...read more.

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