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I will do three different practical experiments to investigate the growth and isolation of bacteria and the characteristics of different microbes. They are:Pouring Streaking Environment

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Practical report Microbial techniques Introduction I will do three different practical experiments to investigate the growth and isolation of bacteria and the characteristics of different microbes. They are: * Pouring * Streaking * Environment Pouring plates Pouring plates can be used to investigate if bacteria can grow on a solidified nutrient agar by following the aseptic techniques carefully. The aseptic techniques used were: * Flaming the neck of the bottle by passing the opening through a Bunsen burner * The lid of the bottle is opened using the small finger of the other hand during procedure to avoid placing the lid on the bench, where it can be contaminated Apparatus Bunsen burner Bottle containing a nutrient agar Petridish Matches Method 1. Remove the bottle containing molten nutrient agar from the hot water. Allow the liquid agar to cool down. 2. light the Bunsen burner and adjust it to blue flame 3. Open the bottle with little finger on the other hand to avoid placing the lid on the bench. ...read more.


This can be avoided by transferring the suspension to the petredish, adding molten agar, then swirling the plate to mix the two liquids. However, even when plate is swirled repeatedly and in directions, the liquids are not mixed as evenly as in the former procedure. Streak plate Streaking plate for single colonies is one of the most important techniques used by microbiologists, since it is used in the initial isolation of a cell culture and in maintaining stock cultures. The aim of this practical is to achieve single colonies at same point on te plate. In this practical assignment, a solidified nutrient agar is used to grow and later identify the bacteria that grew on the streaked agar, using aseptic techniques very carefully. Apparatus Metal loop Solid nutrient agar Culture (E.coli bacteria) Match Bunsen burner Method 1. Take a prepared solidified nutrient agar in a petridish. 2. flame the metal loop, using a Bunsen burner. ...read more.


Spread the sample on prepared agar. 3. Then the sample is left in refrigerator for a week. Results There were 4 types of bacteria identified on the agar in the plate. Discussion and conclusion The results of the practical prove that there are bacteria living nearly everywhere around us. Their populations are quite high where disinfectants are not used, like under the benches, on the surface of the building or even on our working area. Health and safety * Make sure any cuts on exposed parts of the body are covered by waterproof dressings. * Before and after the experiment, worktops must be cleaned with disinfectant solution, so that any previous contamination of microbes is washed away. * Liquids which may contain microorganisms must not squirt quickly into a container as this might form an aerosol and cause the dispersal of spores. * The inoculating loop must be heat- sterilised before and after transferring microorganisms from one medium to another, as may cause unwanted contamination. * Hands should be washed thoroughly with disinfectant before leaving the laboratory. ?? ?? ?? ?? Hani Ali BTEC National diploma in science ...read more.

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