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In my coursework experiment, I am going to investigate the amount of oxygen produced when a source of catalase enzyme is added to hydrogen peroxide, H O .

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Introduction

Biology Coursework Adam Clayton In my coursework experiment, I am going to investigate the amount of oxygen produced when a source of catalase enzyme is added to hydrogen peroxide, H O . In my experiment, I am going to focus on how the concentration of the peroxide affects the rate of reaction i.e. the concentration goes up, the rate of reaction increases. The catalyst is one, which makes the peroxide decompose into water and oxygen. An enzyme is a biological catalyst. They speed up the rate of a reaction however they are not affected themselves whilst doing this, this is why they are catalysts. Each enzyme has an active site. Enzymes all work best at an optimum temperature that is usually body temperature at 37C. If the temperature that the enzyme has to work at gets too high, normally 40C it will start to become denatured and therefore no longer work on its substrate as the active site has changed shape. Also enzymes usually work best at an optimum pH level. Most reactions work better at higher temperatures, this is because molecules move around much quicker. ...read more.

Middle

I would expect 100% to react quickest because it has the most Hydrogen Peroxide molecules in it. With more of these molecules inside the solution, it is more likely that a collision will take place, molecules must collide in order to react. This means that a reaction is more likely to take place, in a shorter time, making the rate of reaction quicker. More collisions are needed because only one in every 10 to the 14 collisions lead to a successful reaction taking place. The more reactions that take place increases the amount of oxygen produced in the shortest time. Method: To find out how the concentration affects the rate of reaction, I shall measure how long it takes to collect 10cm of oxygen. The reaction I am investigating is: 2H O + catalase � 2H O + O This reaction shows that oxygen is being produced. Once all the samples have been prepared and the equipment I will be using is set up (water basin, a bung, a delivery tube, a measuring cylinder, a syringe, and a stopwatch), I will then collect the concentration of Hydrogen Peroxide I will need for the experiment I will be doing. ...read more.

Conclusion

My graph still looks a bit odd, and some of the results could be wrong, however I can still pull some conclusions from this and one is that we can definitely see that with the stronger concentration the more oxygen we are producing. As you can see from the graph, at low substrate concentrations the rate of reaction is directly proportional to the substrate concentration due to the fact that when the substrate concentration is increased the rate of enzyme-substrate collisions (reaction) is equally increased. However, as the substrate concentration rises the rate of reaction will finally reach a maximum level. This is because all the active sites are saturated with substrate. From my results I have found that the higher the concentration of Hydrogen Peroxide, the quicker the reaction rates, producing oxygen. The results I got were what I had expected and predicted, but I got a few anomalous results. The data that I collected was reasonable for the accuracy of my experiment, it was fairly reliable meaning that the results we collected were mostly expected. A major cause for concern on accuracy was the reading of the overall measurement of the oxygen produced. So if these miss-readings were carried out through the whole investigation and so this may be why I have some anomalous results. ...read more.

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