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In my coursework I am investigating the rate of an enzyme action and what factors affect it. Enzymes are biological catalysts.

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Introduction

Factors affecting the rate of enzyme action (preliminary) In my coursework I am investigating the rate of an enzyme action and what factors affect it. Enzymes are biological catalysts. They speed up the chemical reactions which go on inside living things. Without them the reactions would be so slow that life would grind to a halt. The enzyme that we are going to use in the main experiment is called amylase. It is present in the following substances we used in the preliminary experiment: * Carrots * Potato * Apple In this experiment we are going to see which substance reacts better with hydrogen peroxide. Aim: To find out which substance which amylase reacts fastest with hydrogen peroxide. Key variables: As I have already explained above, I am going to change the variable of substance. However, I will keep all of the other variables at a constant level: * Temperature. * Stirring ( I will not stir any of the substances because it will be impossible to stir them exactly the same way) * Amount of substance (cm�) * Concentration of hydrogen peroxide. Prediction: I predict that the higher the concentration of hydrogen peroxide, the more froth will be produced. Scientific Knowledge: I know that the hydrogen peroxide will excite the amylase present in the substance, therefore giving them more energy to make more random collisions. The more random collisions there are, the faster the reaction will be. ...read more.

Middle

The amylase present in the potato reactions with hydrogen peroxide was far more than the carrots or the apples. Therefore, I am going to use potato in my main experiment. Factors affecting the rate of enzyme action (main) In my coursework I am investigating the rate of an enzyme action and what factors affect it. Enzymes are biological catalysts. They speed up the chemical reactions which go on inside living things. Without them the reactions would be so slow that life would grind life to a halt. The enzyme that we are going to use in the main experiment is called amylase. It is present in the following substances we used in the preliminary experiment: * Carrots * Pork( we didn't use this substance due to the fact we already know its capabilities). * Potato * Liver. ( We didn't use this substance due to the fact we already know its capabilities). We saw these substances react with a certain level of pH of hydrogen peroxide. To see the results see "preliminary experiment". However, in this experiment we are going to see the concentration of hydrogen peroxide. This is because we tested what kind of substance was added and we thought that it would be interesting to change the variable of pH. Key Variables: As I have already explained above, I am going to changes the variable of concentration. ...read more.

Conclusion

I will use goggles. To make sure I am safe from harm I will: * Stand up. * Tuck in my chair. * Remove loose clothing (i.e. blazer). * Tuck in my shirt and tie. To make sure that my investigation is a fair test I will not change of the variables only the one which I want to, which is to change the concentration of hydrogen peroxide. Results Concentration Trial 1 (mm) Trial 2 (mm) Trial 3 (mm) 20% 1 2 3 40% 4 4 6 60% 5 6 7 80% 7 6 8 100% 8 8 8 Analysis In my experiment I could have done five trials to make sure that ach of the results we got weren't rogue one's. I could have made better time keeping in the level of froth. I should have kept strict time keeping. Also, my potato could have been cut better. The potato wasn't cut exactly 1cm�. I should have done this because if I had done this, my results would be exact. Conclusion As we can see from my results the higher the concentration of hydrogen peroxide, the more froth is produced. This is because the amylase present in the potato reacts with hydrogen peroxide and reacts with the oxygen (which, in turn, causes frothing). The level of amylase present in potato is ideal because we can measure it with ease. The hydrogen peroxide gives energy to amylase molecules which makes them vibrate and get more collisions. This is what happens in a reaction. Barrett Downing 4CP Miss Pascal, 4S2 Miss Noone ...read more.

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