In this experiment I am trying to find the best conditions for amylase to digest starch. I am going to vary the PH from PH6 to PH9 to see which one digests starch the fastest.

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Introduction:

In this experiment I am trying to find the best conditions for amylase to digest starch. I am going to vary the PH from PH6 to PH9 to see which one digests starch the fastest. This will show which PH enzymes will work best in. I will put 3cm³ of amylase, 1cm³ of starch and 1cm³ of buffer to each test tube. I will heat them in a water bath to 40º. I will then every 2 minutes test the solution for starch using iodine.

Prediction:

I predict that amylase will digest fastest at PH7 because it is the same PH as inside your mouth wear enzymes would be digested.

Equipment:

Test Tubes

Test Tube Rack

Stop Watch

Thermometer

Pipette

Starch

Amylase

Buffer-PH6, PH7, PH8, PH9

Dimple Tray

Preliminary Tests:

I will use these tests to see whether I need to change my amounts of starch, amylase and buffer or the times when I measure the solution.

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Time(mins)  Is Starch Present

       1                        Yes

       2                        Yes

       3                        Yes

       4                        Yes

       5                        Yes

       6                       ...

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