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In this experiment I am trying to find the best conditions for amylase to digest starch. I am going to vary the PH from PH6 to PH9 to see which one digests starch the fastest.

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Introduction

Introduction: In this experiment I am trying to find the best conditions for amylase to digest starch. I am going to vary the PH from PH6 to PH9 to see which one digests starch the fastest. This will show which PH enzymes will work best in. I will put 3cm� of amylase, 1cm� of starch and 1cm� of buffer to each test tube. I will heat them in a water bath to 40�. I will then every 2 minutes test the solution for starch using iodine. ...read more.

Middle

Time(mins) Is Starch Present 1 Yes 2 Yes 3 Yes 4 Yes 5 Yes 6 Yes 7 Yes 8 Yes 9 Yes 10 Yes 11 No The experiment was taking too long so we changed the time when we measured the solution to 2 minutes rather than 1. Test: We first tested PH6: Time(mins) Is Starch Present 2 Yes 4 Yes 6 Yes 8 Yes 10 Yes 12 Yes 14 Yes 16 Yes 18 Yes 20 Yes 22 Yes 24 Yes 26 Yes 28 Yes 30 Yes 32 No PH6 took 32 minutes to digest the starch. ...read more.

Conclusion

Evaluation: I think our results turned out well and produced a clear conclusion. The apparatus we used was not totally accurate but we still got decent results. I think our results could be improved by testing more PH's and testing each PH more than once which didn't get time to do. The chart shows the pattern of time the enzymes took to digest the starch. Conclusion: In conclusion the best PH for amylase to digest starch is PH8 which was not what I predicted although the test may not have been entirely accurate. ...read more.

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