Introduction:
In this experiment I am trying to find the best conditions for amylase to digest starch. I am going to vary the PH from PH6 to PH9 to see which one digests starch the fastest. This will show which PH enzymes will work best in. I will put 3cm³ of amylase, 1cm³ of starch and 1cm³ of buffer to each test tube. I will heat them in a water bath to 40º. I will then every 2 minutes test the solution for starch using iodine.
Prediction:
I predict that amylase will digest fastest at PH7 because it is the same PH as inside your mouth wear enzymes would be digested.
Equipment:
Test Tubes
Test Tube Rack
Stop Watch
Thermometer
Pipette
Starch
Amylase
Buffer-PH6, PH7, PH8, PH9
Dimple Tray
Preliminary Tests:
I will use these tests to see whether I need to change my amounts of starch, amylase and buffer or the times when I measure the solution.