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In this experiment, mung bean seedlings and Brine shrimp eggs were used to study the effect of temperature on the rate of germination of seedlings and the rate of hatching of eggs.

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Introduction

Title : Investigation on the effect of temperature on the development of organisms Abstract : The purpose of carrying out this experiment is to investigate the effect of different temperature on the development of organisms. The effect of increasing temperature on the development of living organisms can be modeled in the laboratory by measuring the germination of plants seedlings and the hatching rate of living organisms. This experiment is to show the effect of rising temperature on the earth or in other words, global warming on the living organisms. It is also to show which temperature is the most suitable for living organisms to live in. Temperature plays an important role on the enzyme activity which in turn affects the whole organism. In different salinity, living organisms also will have a different rate of development. In this experiment, mung bean seedlings and Brine shrimp eggs were used to study the effect of temperature on the rate of germination of seedlings and the rate of hatching of eggs. Three different temperatures that is 20oC, room temperature of 25oC and 30oC were used to investigate the effect of temperature on the development of both mung bean seedlings and Brine shrimp eggs while 1% and 1.5% salt solution were used for the Brine shrimp eggs. The number of seeds germinated, length of the radicles and the number of leaves were measured to determine the rate of development of mung bean seedlings. On the other hand, the number of eggs hatched, number of shrimps alive and mobility of shrimps were observed in order to examine the development of organisms. In conclusion, the optimum temperature for the development of organisms will be the room temperature that is around 25oC and the optimum salinity for the development of Brine shrimp is at 1.5% salt solution. Problem statement: Does global warming affect the rate of development of organisms? What is the effect of increased temperature on the rate of development of organisms? ...read more.

Middle

4.77 0 3 4 3 2 4.40 Mobility of shrimps No Yes Yes Table 3 : The hatching rate of Brine shrimp at room temperature of 25oC in 1% salt solution Time (hours) 24 48 72 Samples 1 2 3 4 5 M (%) 1 2 3 4 5 M (%) 1 2 3 4 5 M (%) No of eggs observed 28 35 98 42 47 - 25 32 78 50 35 - 35 60 23 40 32 - No of eggs hatched 3 7 5 12 8 16.3 5 9 7 13 9 21.8 5 10 12 8 9 26.3 No of shrimps alive 2 4 3 9 9 12.4 3 2 5 10 9 14.1 4 9 11 6 7 22.2 Mobility of shrimps Yes Yes Yes Table 4 : The hatching rate of Brine shrimp at 30oC in 1% salt solution Time (hours) 24 48 72 Samples 1 2 3 4 5 M (%) 1 2 3 4 5 M (%) 1 2 3 4 5 M (%) No of eggs observed 25 33 72 40 26 - 22 45 81 20 18 - 31 40 25 42 48 - No of eggs hatched 4 11 6 12 6 22.1 7 5 10 5 7 23.8 8 7 10 11 8 25.2 No of shrimps alive 3 5 4 10 3 13.8 4 3 5 2 3 11.5 3 1 0 3 1 4.28 Mobility of shrimps Yes No No Table 5 : The hatching rate of Brine shrimp at 25oC in 1.5% salt solution Time (hours) 24 48 72 Samples 1 2 3 4 5 M (%) 1 2 3 4 5 M (%) 1 2 3 4 5 M (%) No of eggs observed 32 51 79 98 25 - 59 28 32 21 56 - 35 41 58 34 24 - No of eggs hatched 9 13 8 5 5 17.8 11 10 6 5 8 22.2 13 8 11 4 12 27.5 No of shrimps alive ...read more.

Conclusion

There might be contamination and affects the result of this experiment. Furthermore, if the beakers containing the Brine shrimp eggs are not stirred properly, this will reduce the accuracy of the experiment as some of the eggs will not have enough water to hatch. Not only that, human error can also result when the numbers of eggs are counted wrongly when observe under the compound microscope. Risk Assessment / Precautions Firstly, utmost care has to be taken care when handling glass wares such as beakers, glass rods and Petri dishes as they are fragile and can be broken easily. We should wash our hands with soap before and after the experiment to take care our personal hygiene. Be extra careful when dealing with sharp apparatus such as the forceps and scissors when cutting the cotton wool and transferring the seedlings into the Petri dish. Furthermore, do make sure that the amount of Brine shrimp eggs that are placed into each beaker is almost the same by using two half teaspoon instead of one full teaspoon which might reduce the accuracy of the number of eggs in each beaker. When measuring the eggs, care needs to be taken as not to destroy the eggs. Do not stir the eggs too hard as the eggs might be destroyed while stirring. Suggestion and Improvements First and foremost, aseptic techniques can be carried out during the preparation of the mung bean seedlings so that the results of the experiment can be improved. Besides that, make sure that the cotton wool is wet thoroughly as this will ensure that the mung beans wil have enough water to germinate. Conclusion : Global warming does affect the rate of development of organisms. As the temperature increases, the rate of development of living organism increases until the optimum temperature that is room temperature of 25oC. Beyond the optimum temperature, the rate of development decreases with further increased in temperature. The rate of development of organism increases with higher salinity of solution until the optimum salinity of not more than 3%. The hypothesis is accepted. ...read more.

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A great deal of work has gone into this study. It is a pity that more than one independent variable is involved. More detail is needed in many places, and more complete explanations would allow a better understanding of reasons for decisions made about the experimental method.

Marked by teacher Adam Roberts 30/07/2013

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