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In this investigation I will be trying to find out what reaction enzymes, from pig's liver, has when it is put with different Concentrations and the temperatures of hydro peroxide.

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Introduction In this investigation I will be trying to find out what reaction enzymes, from pig's liver, has when it is put with different Concentrations and the temperatures of hydro peroxide. Background Information: Enzymes are biological catalysts. They speed up chemical reactions in all living things, and allow them to occur more easily. They are in plant cells and animal cells. Although they work powerfully, enzymes are just chemical molecules, made up of proteins. They are too small to be seen when they are inside cells or when they have been released from them. Each particular enzyme has a unique, 3-dimensional shape shared by all its molecules. Within this shape there is an area called the active site where the chemical reactions occur. Some enzymes help to break down large molecules. Others build up large molecules from small ones. While many others help turn one molecule into another. The fastest enzyme known is called catalase. Catalase breaks the chemical hydrogen peroxide down to water and oxygen. Catalase is found in all cells and protects them from this dangerous waste chemical. Enzymes are sensitive and work best under specific conditions. Each type of enzyme has its own specific optimum conditions that it works best in. ...read more.


Then connect the test tube in the water, with the one with 1ml of enzyme solution in it. Then we will add the hydro peroxide and put the stopper in as quickly as possible so we do not lose any oxygen and then wait for 20secs. We will then measure how much oxygen has been collected in the glass test tube under the water in cm. When we had to do the concentration of the hydro peroxide we will simply keep the amount at 5ml and change the amount of water and hydro peroxide like 4ml water and 1 ml hydro peroxide, 3ml water and 2ml hydro peroxide etc. When we do the temperatures of 40�C and 60�C we will have to put the test tubes with hydro peroxide solution and enzymes in a water bath, so to maintain the temperature we need. When we do 15�C we will have to add ice to our tub and put the test tube in that until it is at the temperature we need. For the potato enzyme experiment we did the same apart from replacing the liver with pieces of potato and grinding that up with sand and water instead. ...read more.


The 60�c potato test the first two outcomes were two small to measure with what we were measuring with. When we ran out of liver enzyme we did not get more liver because it would not have been the same liver and would not have had the same animal age, shelf life or had the same amount or strength of enzymes. If I was to do this experiment again I would collect 5 pieces of data for each one instead of 3 this would give us more back up if one result was wrong and we could have seen if it fitted into the pattern. I would also make sure I have enough enzyme solution to do all the experiments. I would also have done each test 3 times to get an average. If we wanted to really be accurate with how much oxygen was produced we would have used a gas syringe but we did not have access to these. Our results showed that the higher the temperature the less oxygen produced I don't think this is right. To extend the experiment we could change some of the control variables. Some of these would be hard to do like the pH. All together I think we could improve what we did a lot. ...read more.

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