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In this investigation we are going to measure the effect of temperature change in a reaction between catalase and Hydrogen Peroxide.

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Introduction

Biology Coursework- Investigation on the Effect of Temperature on Catalase Aim: In this investigation we are going to measure the effect of temperature change in a reaction between catalase and Hydrogen Peroxide. Introduction Catalase is an enzyme that's found in living things, it changes hydrogen peroxide into water and oxygen. In our investigation liver will be the source of catalase, we will measure the amount of time it takes for the catalase to complete its reaction with Hydrogen Peroxide and the effect of temperature on the rate of reaction. We will know when it's finished because there will be little oxygen produced and the fizzing will have stopped and there will not be much oxygen given out. The reaction is between catalase and hydrogen peroxide(H2O2). (Space for formula and diagram) Preliminary Work Investigating the effect of temperature on the action of Enzymes. Using Pepsin and Egg white.This experiment was done to give us an idea for the prediction in our main experiment, we tested using an enzyme to give us a general idea of the effect of temperature on an enzyme, so that we could plan the range of temperature that we would use in our main experiment. Apparatus ? 6 Test tubes ? 1 Stop clock ? 1 Test tube rack ? Kettle ? Thermometer ? Beaker ? 5ml egg white suspensor ? 2ml acidified pepsin Method ? Clearly, label each test tubes, 1-6. ? Pour 5ml of egg white suspension into the test tubes 1, 3 & 5. ? Pour 2ml acidified pepsin into the test tubes 2, 4 & 6. ? Leave the test tubes 1& 2 in a test tube rack at room temperature for 5 minutes (make a note of room temperature) ...read more.

Middle

Prediction By referring to our preliminary results I can predict that the higher the temperature of the water the faster the rate of reaction. I think this because the optimum temperature for catalase is probably 37�C (body temperature) and after that temperature it pretty much denatures, hence why the reaction is so fast. 37�C is when catalase functions at its best. Due to the collision theory we know that this happens because as the temperature increases the molecules have more kinetic energy, therefore more collisions happen resulting in more reactions. However when the temperature gets too high the rate of reaction slows down because the active site of the enzyme has been denatured and the substrate doesn't fit any more in order for there to be a reaction. Results For 85�C: Time/seconds Test 1 Vol of O2/cm� Test 2 Vol of O2/cm� Test 3 Vol of O2/cm� Test 4 Vol of O2/cm� Average Vol of O2/cm� 0 0 0 0 0 0 5 5 4 8 8 4.5 10 6 6 13 9 6 15 8 7 15 9 7.5 20 9 9 16 9 9 25 9 9 19 12 9 30 9 10 20 15 9.5 35 10 10 20 15 10 40 10 10 22 16 10 45 10 10 25 17 10 50 10 11 27 18 10.5 55 11 11 30 19 11 60 11 11 30 20 11 Observation The highlighted results are outliers because they were both very different to each other and their rate of reaction faster than others , instead we used two different more similar results to calculate the average and excluded the outliers. ...read more.

Conclusion

Another reason for the results sometimes not following a specific patter is the liver drying out after being left for a while meant different results to what you would have collected from a fresher piece of liver. Also in our resukts for 22�C a faulty gas syringe meant that less CO2 was collected in the syringe, was exluded this result, along with all other outliers, from the averages. Genarally my experiments went very well, I had not trouble with any of the equiptment (accpet from the gas syringe) and thought that every thing went realtively smoothly. Although the set up of equiptment was quite compliacted it was worth it in order to achiev results that were as accurate as posible. I think that by using the gas syringe instead of other methods (such as the beehive shelf) I managed to obtain results that were as concise as possible. However if I were to repeat the experiment I would have repeated each set of results at least 6 times each, this would make sure the results are very accurate and leave less room for human error. Another thing I would do is use a bigger range of temperatures, as well as the ones I did do I would test one that is nearer to 37�C so that I could get a better idea of what the optimum temperature is. In conclusion I think that my results are reliable, although they had quite big ranges, their averages even them out and got them on to the pattern that they were meant to follow. The experiments results agreed with my prediction (the higher the temperature of the water the faster the rate of reaction) which means that ,despite the initial large range of results, they are reliable. ...read more.

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