• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigate concentration of amylase to see how fast it breaks down.

Extracts from this document...

Introduction

INVESTIGATION Aim To investigate concentration of amylase to see how fast it breaks down. Scientific Knowledge An enzyme is a biological catalyst. A catalyst enables substances to react more quickly. Atoms break and form easily which are between bonds which are helped by catalysts. The particles need less energy, so the proceeds more quickly. For example, catalysts are now used in car exhaust systems. Car fumes contain poisonous carbon monoxide and nitrogen oxide. These pollutants react together very slowly to form, carbon dioxide and nitrogen which are not poisonous. Carbon monoxide + nitrogen oxide --> Carbon dioxide + Nitrogen 2Co + 2No --> 2Co2 + N2 When the exhaust's fumes pass over the platinum, the carbon monoxide and nitrogen oxide react together very rapidly. However, none of the platinum is used up during the reaction. The mass of platinum is the same after the reaction as before. The platinum has acted as catalyst. Therefore we state a catalyst as a substance which alters the rate of a reaction without being used up. (Reference: revise GCSE Single and Double award) There are different factors that affect the reaction rates. ...read more.

Middle

The reason it cannot do this is, because the shape of it is different to the substance. The downside of this process is that only a limited amount can fit at the active sites which can makes the process itself slow. Apparatus Spotting palette 1% starch Concentrated solution 0.25%-1.25% Pipettes Iodine Test tube Test tube rack Timer Method 1. Put 2 drops of iodine in to each dimple on the spotting tray. Keep pipettes separated to prevent cross-contamination and making sure you use the same pipette for the same job. 2. Collect water from the water bath and place the enzymes on it for a couple of minutes to get the enzymes working. 3. In the meantime, mix starch and amylase of 0.25%. 4. Put 2 drops of solution into the dimples which contain iodine. Start the timer. Every 20 seconds put the concentrated solution in to the dimple containing iodine. 5. Watch for the change of colour; form brown to black. Stop the timer once the colour has changed. 6. Record the time taken for the starch to convert in to sugar. ...read more.

Conclusion

To prevent cross- contamination, we could have used labels on the pipettes and other instruments. We could also have special pipettes to allow certain amount of drops. The other problem was time, if we had enough time, we would be able to repeat the procedures enough to get a decent average. For a further investigation and to support my conclusion, I will have a second method. To produce this evidence, I will have to produce an investigation to produce the similar results. The things that we need to keep in mind are that we had problems with the colour. To resolve this, I will change the enzyme. The substrate I will use is a chemical metabolic waste known as hydrogen peroxide. It is produced by every cell. It is catalyse that reacts with the toxin to make water and oxygen. The chemical formula is: 2h2o2-->H2o+o2 This will prove my conclusion because as the enzyme reacts with the hydrogen peroxide, two substances are created. They are water and oxygen. The oxygen will go through the pipe in to the water and up the measuring cylinder. This will show is how much oxygen is collected and measure it with the cylinder of how much oxygen is collected up. CREATED BY UMAR ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Effect Of Substrate Concentration On The Activity Of Catalase

    The trial experiment showed me how quickly each concentration of hydrogen peroxide produced the amount of oxygen gas in one minute. I also learnt that slow pressure is needed with the small gas syringe because too much pressure causes the catalase to spill out.

  2. The Iodine Clock Investigation

    Method The method used was the same as in the first set of trials. Results a) Varying the concentration of KI. Concentrations used: H2O2 - 2.5M H2SO4 - 1.0M Na2S2O3 - 0.05M Concentration of KI (M) Time taken for reaction to reach End point. (secs) 1.0 8.22 0.5 17.55 b)

  1. Enzymes - show how substrate concentration affects the rate of reaction for an enzyme ...

    xi n Hence x = 6.8 6 = 1.133333333 = 1.13 y = ? yi n So y = 0.6769 6 = 0.112816666 = 0.113 � = Sxy Sxx So therefore � = 0.97088 9.68 = 0.10029752 = 0.100 � = y - �x Hence � = 0.113 - (0.100 � 1.13)

  2. Magnesium Oxide

    the table which cause the balance to be unstable for quite some time. Why this is affecting our results is because we cannot be sure if the readings we have recorded are reliable because we recorded them while the balance was unstable.

  1. How fast is starch broken up by the catalyst amylase.

    - The amount of starch and amylase used is 10ml; we used a measuring cylinder to help. - We poured the solutions into separate test tubes and placed them in a beaker of water. - To change the temperature we either poured ice/cold water to bring it down, and hot

  2. Investigating the effect of enzyme concentration on the hydrolysis of starch with amylase.

    Amylose forms a helical complex with iodine giving a characteristic blue colour. Amylopectin is another composition of starch; it has a polymeric branched structure. Amylopectin has a-D-1, 4 bonds, linear segments and amylopectin molecule has a-D-1, 6 bonds, which occur every 20-30 anhydrous units.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work