A chemical equation for photosynthesis is:
Light energy.
6CO2 + 6H2O C6H12O6 + 6O2
Light energy.
Carbon dioxide + Water Glucose + Oxygen.
To keep the equation simple, glucose is shown as the food compound produced.
Aim
The aim of this experiment is to investigate how light intensity affects the rate of photosynthesis. The other factors that I could have investigated were:
Temperature and the amount of carbon dioxide needed in order for photosynthesis to take place.
Hypothesis:
My hypothesis is that as the intensity of light is increases so does the rate of photosynthesis. The reason behind this is because light is an essential source for photosynthesis to take place. In order to test this I will use a lamp, which will be the source of light and set it up as shown in the diagram overleaf. I will try to make it a fair test. This light energy will be absorbed into the pigment of the leaf. Also found in the leaf is a part called chlorophyll, which is essential within the plant.
The chlorophyll pigments occur in the green plant cells, the stacked discs of membranes found in the chloroplasts. Unlike most pigments of plants chlorophyll is largely insoluble in water, but may be extracted using organic solvents. This light absorbed which is then transformed into chemical energy through a series of reaction the process but of course this energy cannot be used in photosynthesis. The result of the light energy is absorbed by chlorophyll in solution is re-mitted as red fluorence.
Method
- Collect the above apparatus. Make sure they are washed in clean water using detergent as otherwise bubbles may stick to the apparatus.
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Using a measuring tube collect 10cm3 of sodium hydrogen carbonate.
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Add it to250cm3of water (H2O)
The reason for this is to maintain the level of carbon dioxide (CO2)
- Collect 5cm of Canadian pondweed (Elodea)
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Place this into a beaker but DO NOT PUT IT IN THE WATER AND NaHCO3 SOLUTION JUST YET!
- The reason for this is because the rest of the apparatus has not been set up and it will make the results unreliable.
- Attach the photosynthometer to the stand + clamp.
- Set it up as shown in the diagram.
- Keep the metre stick in a secure place avoid it from moving. (A suggestion you keep it stuck down by using a bit of masking tape at the bottom of it.)
- Have the lamp ready to switch on. BUT NOT YET!
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When ready put Elodea in the solution (water and NaHCO3) and attach it to the micro-burette.
- Turn on the lamp, which should be at the appropriate distance on the meter stick.
- NOTE: lamp and stopwatch should be switched at the same time.
- Take the results after every minute.
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Repeat the last 3 steps 10 times. However change water every time, use same amount of NaHCO3 (10cm3 ). Finally keep the temperature the same to make it a fair test.
Apparatus
Beaker
Water- 250cm3
Elodea
Thermometer
Metre stick
Lamp bulb –60W
Stopwatch or clock with a second hand.
Measuring cylinder
Photosynthometer
Capillary tube- graduated.
Clamp + stand
Tripod to support the beaker containing
Elodea.
10cm3 sodium hydrogen carbonate solution
Micro-burette (that is washed in clean water to avoid the bubbles from sticking to it.)
Diagram:
Results table.
The results from the experiment will be recorded 10 times.
Evaluation.
In order to prove my hypothesis, a graph was plotted. The results revealed that the distance of the lamp from the Elodea was 100cm; the number of bubbles per minute was 13. When the distance was 80cm, the number of bubbles per minute appeared to be 12. I would have assumed that the number of bubbles in this case would have been higher as stated in my hypothesis. The rest of my results were as I had expected.
To ensure that the experiment was accurate the lamp had to be moved 20cm from its original 100cm every minute an then stopped it when it reached 0cm.therefore I had to make sure that I monitored the time accurately. The test was repeated 10 times in order to make it fair. This gave me an average. Another way of making it a fair test would be to use oxygen to keep the plant alive- this is indirect of photosynthesis. By using oxygen I can use the same piece of Elodea several times without destroying it when using glucose.
As you can see by referring to the graph only 6 out of 10 results were recorded, as it was clear that my initial hypothesis was correct.
Instead of using the sophisticated apparatus: photosynathermetre and the graduated capillary tube I could have simply used a beaker, test tube, stop watch, a lamp, a metre stick and the same solution (water and NaCHO3). However this experiment would have been inaccurate because I would have had to count the bubbles myself and measure the gas quantity. The other disadvantages I may encounter would be the following:
- When the lamp is at its furthest distance (100cm) from the Elodea the bubbles are going to reduce by quantity and size, therefore the bubbles maybe too small to see which would have made them difficult to count as a result.
- The lamp could be too close to the test tube and heat up the water quickly making the temperature to be inaccurate and the rate of photosynthesis would vary.
- When the lamp is at its closest distance (0cm) from the Elodea the bubbles are going to be rapidly produced therefore once again making it difficult to count.
- If the lamp heated the water up, the temperature would have increased giving me two limiting factors: temperature and light intensity, instead of just one. This would have gone against my hypothesis.
- The whole experiment would not be as accurate as the photosynthometer.
The main advantage in using the above apparatus’ has been that it is easy to set-up and use.