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Investigate if the rate of reaction between Hydrogen peroxide and the enzymes on a potato would increase if the surface area was increased too.

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Introduction

Enzymes Coursework Aim: To investigate if the rate of reaction between Hydrogen peroxide and the enzymes on a potato would increase if the surface area was increased too. An enzyme is any one of many specialized organic substances, composed of polymers of amino acids that act as catalysts to regulate the speed of the many chemical reactions involved in the metabolism of living organisms. Enzymes are large proteins that speed up chemical reactions. In their globular structure, one or more polypeptide chains twist and fold, bringing together a small number of amino acids to form the active site, or the location on the enzyme where the substrate binds and the reaction takes place. Enzyme and substrate do not bind if their shapes do not match exactly. This ensures that the enzyme does not aid in the wrong reaction. The enzyme itself is unaffected by the reaction. When the products have been released, the enzyme is ready to bind with a new substrate. Enzymes are extraordinarily efficient. Minute quantities of an enzyme can accomplish at low temperatures what would require violent conditions and high temperatures by ordinary chemical means. Each enzyme is selectively specific for the substance in which it causes a reaction and is most effective at a temperature peculiar to it. ...read more.

Middle

It is very important that there are no air bubbles left in the measuring cylinder. After that I will place one end of the tube inside the measuring cylinder making sure that the end without the cork is place in. Now I have to measure 30ml of Hydrogen peroxide with the other measuring cylinder and pour it all into the conical flask. Finally its just a case of placing the strip of potato into the flask and putting the cork with the tube on top of the flask and start timing. This is how the experiment works, when the reaction takes place oxygen is released as a product of it, as it is released it travels along into the tube and goes straight into the measuring cylinder. When the oxygen enters the cylinder it displaces the water in it and collects at the top of the cylinder, this happens because the oxygen is lighter than the water and can push it aside so that it can reach the top. Once the oxygen is collected we can measure how much has collected in it with the markings on the cylinder. I am going to vary the surface area of the potato through out the experiment, and control all of the other variables. ...read more.

Conclusion

This might have made the results seem larger than they should be. Another is human error. To measure the hydrogen peroxide I had to use the measuring cylinder which involves using the human eye to get an accurate reading, the human eye can sometimes be deceiving, the wrong amount of hydrogen peroxide might have been used, this would have affected the results. A combination of human error and faulty equipment could render the experiment a failure. Sometimes people rush things and takes down a wrong reading and also faulty equipment can sometimes give wrong readings. The method of the experiment could probably also be improved to obtain more reliable results. Due to the nature and convenience of the experiment, it could be easily modified to investigate another factor. I investigated the Surface area when I could have investigated the Concentration or the temperature, by investigating them I could have got better results but controlling the temperature would have been really difficult, putting in all that effort might not have been worth it. The evidence that I obtained is sufficient enough to support the conclusions I have made. Because I conducted my experiment as accurately as I could with the method I used. Rumel Ahmed 3873 Page 1 of 6 ...read more.

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