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Investigate the affect of one or more factor on the rate of reaction, when the enzyme peroxidase (catalase) breaks down Hydrogen Peroxide.

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Introduction

Year 10 Assessed Biology Practical Investigate the affect of one or more factor on the rate of reaction, when the enzyme peroxidase (catalase) breaks down Hydrogen Peroxide Introduction: Peroxidase can be extracted from many plant and animal tissues. The extract that you are using was produced by crushing potatoes tubers in water, then filtering and centrifuging the extract produced to remove the cell debris. Hydrogen peroxide is easily broken down by the enzyme peroxidase as follows: - 2H2O2 2H2O + O2 Enzymes are important ...continue... Some properties of enzymes: 1. They are always Proteins 2. They are specific in their actions. (This means that each enzyme controls one particular reaction, or type of reaction.) 3. They can be used over and over gain. (This is because they are not altered by the reaction in which they take part.) 4. They are destroyed by heat. ...read more.

Middle

Put 40 ml of Hydrogen Peroxide Solution in to the 50ml beaker. 3. Dip the filter paper, using the forceps, in to the Potato Extract. Remove. Shake, to rid off any excess liquid. 4. Hold the paper, with the extract on it, 1cm above the Hydrogen Peroxide Solution and drop it in. 5. As soon as the filter paper hits the bottom of the beaker, start timing. 6. As soon as the paper reaches the top again, stop timing. 7. Record the time it took to reach the top from when it hit the bottom. 8. Remove the paper. 9. Dot he experiment exactly the same BUT change the PH of the next Extract you will be using. Safety Note: Hydrogen Peroxide is corrosive. You should wear safety spectacles when working with the solution. If any comes in contact with your skin or clothes wash immediately under cold water. ...read more.

Conclusion

PH8 6.07secs. 7.02secs. 5.47secs. 6.19secs. In this preliminary experiment I carried out the experiment as if I were doing the real one, so it is exactly the same. After I collected my results I had realized that there was no reaction for the H2O2 with the PH4 Peroxidase. I think this is because the enzyme is de-natured by the acidity of itself. This is backed up by the background information in the introduction. I have only tested half the of the PH's so I could only get a rough idea of what the results would be like. I also only tested the even numbers because they were the numbers that were around the middle so I could get a good idea of what the rest of the results would look like. Preliminary Graph: Results: 1st 2nd 3rd Average PH4 NA NA NA NA PH5 5.79secs. 5.28secs. 4.06secs 5.04secs. PH6 8.79secs. 8.70secs. 5.96secs. 7.81secs. PH7 6.37secs. 6.34scs. 7.05secs. 6.58secs PH8 6.53secs. 5. PH9 ...read more.

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